Presented orally at the 116th Triological Society Meeting Combined Otolaryngology Spring Meetings, Orlando, Florida, U.S.A., April 13, 2013.
Facial Plastics and Reconstructive Surgery
Improved wound healing of postischemic cutaneous flaps with the use of bone marrow-derived stem cells
Article first published online: 19 JUL 2013
© 2013 The American Laryngological, Rhinological and Otological Society, Inc.
Volume 124, Issue 3, pages 642–648, March 2014
How to Cite
Hu, M., Ludlow, D., Alexander, J. S., McLarty, J. and Lian, T. (2014), Improved wound healing of postischemic cutaneous flaps with the use of bone marrow-derived stem cells. The Laryngoscope, 124: 642–648. doi: 10.1002/lary.24293
The authors have no funding, financial relationships, or conflicts of interest to disclose.
- Issue published online: 18 FEB 2014
- Article first published online: 19 JUL 2013
- Accepted manuscript online: 1 JUL 2013 07:16AM EST
- Manuscript Accepted: 13 JUN 2013
- Manuscript Revised: 2 JUN 2013
- Manuscript Received: 27 MAR 2013
- Bone marrow stromal cells;
- stem cells;
- cutaneous flaps;
- free tissue transfer;
- wound healing;
- Level of Evidence: NA
To determine if the intravascular delivery of mesenchymal stem cells improves wound healing and blood perfusion to postischemic cutaneous flap tissues.
Randomized controlled study.
A murine model of a cutaneous flap was created based on the inferior epigastric vessels. Mice (n = 14) underwent 3.5 hours of ischemia followed by reperfusion. Bone marrow stromal cells (BMSCs) 1 × 106 were injected intravenously. Wound healing was then assessed measuring percent flap necrosis, flap perfusion, and tensile strength of the flap after a period of 14 days. Localization of BMSCs was determined with radiolabeled and fluorescent labeled BMSCs.
Postischemic cutaneous flap tissues treated with BMSCs demonstrated significantly less necrosis than control flaps (P <0.01). Beginning on postoperative day 5, BMSC-treated flaps demonstrated greater blood perfusion than untreated flaps (P <0.01). Tensile strength of BMSC-treated cutaneous flaps was significantly higher (P <0.01), with a mean strength of 283.4 ± 28.4 N/m than control flaps with a mean of 122.4 ± 23.5 N/m. Radiolabeled BMSCs localized to postischemic flaps compared to untreated tissues (P = 0.001). Fluorescent microscopy revealed incorporation of BMSCs into endothelial and epithelial tissues of postischemic flaps.
This study demonstrates that the intravascular delivery of BMSCs increases wound healing and promotes flap survival following ischemia-reperfusion injury of cutaneous tissue flaps. Laryngoscope, 124:642–648, 2014