Cytokine correlation between sinus tissue and nasal secretions among chronic rhinosinusitis and controls

Authors

  • Samuel L. Oyer MD,

    1. Division of Rhinology&Sinus Surgery, Department of Otolaryngology–Head and Neck Surgery, Medical University of South Carolina, Charleston, South Carolina
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  • Jennifer K. Mulligan PhD,

    1. Division of Rhinology&Sinus Surgery, Department of Otolaryngology–Head and Neck Surgery, Medical University of South Carolina, Charleston, South Carolina
    2. Research Service, Ralph H. Johnson VA Medical Center, Charleston, South Carolina
    3. Rhinology Division, Department of Otolaryngology–Head and Neck Surgery, Stanford Medical Center, Stanford, California, U.S.A.
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  • Alkis J. Psaltis MD, PhD,

    1. Rhinology Division, Department of Otolaryngology–Head and Neck Surgery, Stanford Medical Center, Stanford, California, U.S.A.
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  • Oswaldo A. Henriquez MD,

    1. Division of Rhinology&Sinus Surgery, Department of Otolaryngology–Head and Neck Surgery, Medical University of South Carolina, Charleston, South Carolina
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  • Rodney J. Schlosser MD

    Corresponding author
    1. Division of Rhinology&Sinus Surgery, Department of Otolaryngology–Head and Neck Surgery, Medical University of South Carolina, Charleston, South Carolina
    2. Research Service, Ralph H. Johnson VA Medical Center, Charleston, South Carolina
    • Send correspondence to Rodney J. Schlosser, MD, MSC 550, 135 Rutledge Ave. Charleston, SC 29425. E-mail: schlossr@musc.edu

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  • Funded in part by grants to R.J.S. (grant 113039) and J.K.M. (grant 092401) from the Flight Attendants Medical Research Institute, and to R.J.S. from the Department of Veterans Affairs. R.J.S. is a consultant for BrainLAB, Olympus, and Sunovion and received previous grant support from Medtronic, Arthrocare, NeilMed, and the speaker's bureau for NeilMed. The authors have no other funding, financial relationships, or conflicts of interest to disclose.

Abstract

Objectives/Hypothesis

Compare cytokine levels in sinus tissue to sinus secretions from controls and chronic rhinosinusitis patients.

Study Design

In vitro.

Methods

Polyurethane foam sponges were placed into middle meati of patients with chronic rhinosinusitis without nasal polyps (CRSsNP), with polyps (CRSwNP), and controls. Sinus biopsies were then taken from the same location. Protein levels of tumor necrosis factor-alpha (TNF-α), interferon-γ (IFN-γ), and interleukins (IL) 2, 4, 6, 8, 10, and 17A were measured via cytometric bead assay for each sample. Protein values from sinus tissue and secretions were compared with Pearson's correlation between samples as well as one-way ANOVA with posthoc t test between groups.

Results

Samples from 43 patients in total were examined. Mucus was measured from 10 controls, 11 CRSsNP and 10 CRSwNP, and sinus tissue was measured from 10 controls, 15 CRSsNP and 10 CRSwNP. IL-8 and IFN-γ levels were outside of the detectable range of the assay. Levels of secreted IL-2, 4, 6, 10, and 17A correlated with tissue levels (P < 0.05 for all, r > 0.49) while TNF-α did not (P = 0.71). CRSsNP had elevated mucus levels of IL-2, 4, 6, 10, and 17A compared to controls. CRSwNP had elevated mucus levels of IL-4, 6, 10, 17A, and TNF-α compared to controls.

Conclusions

Cytokine levels in sinus secretions correlate with levels in sinus tissue and are elevated in CRS versus control based on Th1/Th2 skewing.

Level of Evidence

N/A (in vitro). Laryngoscope, 123:E72–E78, 2013

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