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Recovery of vibratory function after vocal fold microflap in a rabbit model

Authors

  • Tsuyoshi Kojima MD, PhD,

    1. Department of Otolaryngology, Vanderbilt Bill Wilkerson Center, Vanderbilt University School of Medicine, Nashville, Tennessee, U.S.A
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  • Joshua R. Mitchell MD,

    1. Department of Otolaryngology, Vanderbilt Bill Wilkerson Center, Vanderbilt University School of Medicine, Nashville, Tennessee, U.S.A
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  • C. Gaelyn Garrett MD,

    1. Department of Otolaryngology, Vanderbilt Bill Wilkerson Center, Vanderbilt University School of Medicine, Nashville, Tennessee, U.S.A
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  • Bernard Rousseau PhD

    Corresponding author
    1. Department of Otolaryngology, Vanderbilt Bill Wilkerson Center, Vanderbilt University School of Medicine, Nashville, Tennessee, U.S.A
    2. Department of Hearing and Speech Sciences, Vanderbilt Bill Wilkerson Center, Vanderbilt University School of Medicine, Nashville, Tennessee, U.S.A
    • Send correspondence to Bernard Rousseau, PhD, Vanderbilt University Bill Wilkerson Center for Otolaryngology and Communication Sciences, 1313 21st Avenue South, Room 602, Nashville, TN 37232-4480. E-mail: bernard.rousseau@vanderbilt.edu

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  • This paper received first place in the 2013 American Laryngological Association Poster Competition at COSM in Orlando, Florida, U.S.A., April 10–11, 2013.

  • This work was supported by NIH grant R01 DC 011338-01 from the National Institute of Deafness and Other Communication Disorders (NIDCD). The authors have no other funding, financial relationships, or conflicts of interest to disclose.

Abstract

Objectives/Hypothesis

The purpose of this study was to evaluate the return of vibratory function and restoration of vibration amplitude and symmetry after vocal fold microflap surgery.

Study Design

Prospective in vivo animal model.

Methods

Microflap surgery was performed on 30 New Zealand white breeder rabbits. The left vocal fold received a 3-mm epithelial incision and mucosal elevation, while the contralateral vocal fold was left intact to serve as an internal control. Quantitative analysis of amplitude ratio and lateral phase difference were measured using high-speed laryngeal imaging at a frame rate of 10,000 frames per second from animals undergoing evoked phonation on postoperative days 0, 1, 3, 5, and 7.

Results

Quantitative measures revealed a significantly reduced amplitude ratio and lateral phase difference on day 0 after microflap. These impairments of vibratory function on day 0 were associated with separation of the vocal fold's body-cover layer. Amplitude ratio increased significantly by day 3 after microflap, with further increases in vibration amplitude on days 5 and 7. While the amplitude ratio improved significantly on day 3, lateral phase difference decreased significantly on day 3, and returned to normal on days 5 and 7.

Conclusions

High-speed laryngeal imaging was used to investigate the natural time course of postmicroflap recovery of vibratory function. Results revealed the restoration of vibration amplitude and lateral phase difference by days 3 to 7 after microflap. The time period of improved vibratory function observed in this study coincides with the end of the well-documented inflammatory phase of vocal fold wound repair.

Level of Evidence

N/A. Laryngoscope, 124:481–486, 2014

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