Selective plane illumination optical and optoacoustic microscopy for postembryonic imaging (pages L29–L34)
Hsiao-Chun Amy Lin, Andrei Chekkoury, Murad Omar, Tobias Schmitt-Manderbach, Benno Koberstein-Schwarz, Timo Mappes, Hernán López-Schier, Daniel Razansky and Vasilis Ntziachristos
Article first published online: 8 SEP 2015 | DOI: 10.1002/lpor.201500120
Intravital imaging of large specimens is challenging for postembryonic studies. While selective plane illumination microscopy (SPIM) has been widely used to volumetrically visualize organisms, its performance quickly deteriorates for opaque tissues. To overcome this limitation, SPIM was combined with optoacoustic imaging in a hybrid system. A juvenile zebrafish was imaged, revealing high-resolution results over extended depths of scattering content. This method may enable future visualization of opaque organisms throughout their entire development.