Influence of low level laser therapy on wound healing and its biological action upon myofibroblasts
Article first published online: 25 FEB 2003
Copyright © 2003 Wiley-Liss, Inc.
Lasers in Surgery and Medicine
Volume 32, Issue 3, pages 239–244, March 2003
How to Cite
Medrado, A. R.A.P., Pugliese, L. S., Reis, S. R. A. and Andrade, Z. A. (2003), Influence of low level laser therapy on wound healing and its biological action upon myofibroblasts. Lasers Surg. Med., 32: 239–244. doi: 10.1002/lsm.10126
- Issue published online: 25 FEB 2003
- Article first published online: 25 FEB 2003
- Manuscript Accepted: 20 AUG 2002
- low-level laser;
- skin wounds
Background and Objective
In re-evaluating the effects of laser therapy in wound healing, the role of extracellular matrix elements and myofibroblasts, was analyzed.
Study Design/Materials and Methods
Cutaneous wounds were inflicted on the back of 72 Wistar rats. Low level laser was locally applied with different energy densities. Lesions were analyzed after 24, 48, 72 hours and 5, 7, and 14 days. Tissues were studied by histology, immunohistochemistry, and electron microscopy.
In treated animals, the extent of edema and the number of inflammatory cells were reduced (P < 0.05), but the amount of collagen and elastic fibers appeared slightly increased. Desmin/smooth muscle alpha-actin-phenotype myofibroblasts were statistically more prominent on the 3rd day after surgery (P < 0.05) in treated wounds than in controls. Treatment with a dosage of 4 J/cm2 was superior to that with 8 J/cm2.
Laser therapy reduced the inflammatory reaction, induced increased collagen deposition and a greater proliferation of myofibroblasts in experimental cutaneous wounds. Lasers Surg. Med. 32:239–244, 2003. © 2003 Wiley-Liss, Inc.