Photodynamic Therapy of oral dysplasia with topical 5-aminolevulinic acid and light-emitting diode array

Authors

  • Jui-Chang Tsai MD, PhD,

    1. Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, Taipei 100, Taiwan
    2. Center for Optoelectronic Biomedicine and College of Medicine, National Taiwan University, Taipei 100, Taiwan
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  • Chun-Pin Chiang BDS, DMSc,

    1. School of Dentistry, College of Medicine, National Taiwan University, Taipei 100, Taiwan
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  • Hsin-Ming Chen BDS, DMSc,

    1. School of Dentistry, College of Medicine, National Taiwan University, Taipei 100, Taiwan
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  • Sheng Bang Huang MS,

    1. Opto-Electronics & Systems Laboratories, Industrial Technology Research Institute, Hsinchu 310, Taiwan
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  • Chun Wei Wang MS,

    1. Opto-Electronics & Systems Laboratories, Industrial Technology Research Institute, Hsinchu 310, Taiwan
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  • Ming I. Lee MD,

    1. Biomedical Engineering Center, Industrial Technology Research Institute, Hsinchu 310, Taiwan
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  • Yih-Chih Hsu PhD,

    1. Biomedical Engineering Center, Industrial Technology Research Institute, Hsinchu 310, Taiwan
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  • Chin-Tin Chen PhD,

    Corresponding author
    1. Center for Optoelectronic Biomedicine and College of Medicine, National Taiwan University, Taipei 100, Taiwan
    2. Graduate Institute of Oral Biology, College of Medicine, National Taiwan University, Taipei 100, Taiwan
    • Center for Optoelectronic Biomedicine, College of Medicine, National Taiwan University, No 1, Jen-Ai Road, Section 1st, Taipei 100, Taiwan.
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  • Tsuimin Tsai PhD

    Corresponding author
    1. Graduate Institute of Biomedical Materials, Taipei Medical University, Taipei 110, Taiwan
    • Graduate Institute of Biomedical Materials, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan.
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Abstract

Background and Objectives

In Taiwan, more than two million people have the betel quid (BQ) chewing habit which is a risk factor related to premalignant lesion and squamous cell carcinoma of oral cavity. We developed a light-emitting diode (LED) array combined with topical 5-aminolevulinic acid (ALA) for photodynamic therapy (PDT) and evaluated its effectiveness for the treatment of oral lesions.

Study Design/Materials and Methods

We compared the ALA-PDT effect of the homemade LED array to that of a commercial light source on cultured Ca9-22 human gingival carcinoma cells and the DMBA-induced hamster buccal pouch carcinoma model. Furthermore, we treated several patients having an oral lesion using a topical ALA delivery system and the LED array.

Results

The LED array light source was as effective as the commercial light source for ALA-PDT in cultured Ca9-22 cells with LD50 of 4.5 and 4.3 J/cm2, respectively, using an MTT assay. This light source was also effective in the DMBA-induced hamster buccal pouch carcinoma model, and in the patients of oral leukoplakia.

Conclusions

ALA-PDT is effective for premalignant lesions such as mucosal dysplasia and carcinoma in situ of oral cavity. Good results could be obtained by using the homemade LED array as light source. The LED array has the advantages of low cost, high reliability, and portability. It is safe, convenient and easy to use for the treatment of oral dysplasia. Lasers Surg. Med. 34:18–24, 2004. © 2004 Wiley-Liss, Inc.

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