Original Article

In vivo multiphoton fluorescence imaging: A novel approach to oral malignancy

  1. Petra Wilder-Smith DDS, PhD1,*,
  2. Kathryn Osann PhD2,
  3. Nevine Hanna BS1,
  4. Naglaa El Abbadi BS1,
  5. Matt Brenner MD2,
  6. Diana Messadi DDS, DMSc3,
  7. Tatiana Krasieva PhD1

Article first published online: 20 AUG 2004

DOI: 10.1002/lsm.20079

Issue

Lasers in Surgery and Medicine

Lasers in Surgery and Medicine

Volume 35, Issue 2, pages 96–103, August 2004

Additional Information

How to Cite

Wilder-Smith, P., Osann, K., Hanna, N., Abbadi, N. E., Brenner, M., Messadi, D. and Krasieva, T. (2004), In vivo multiphoton fluorescence imaging: A novel approach to oral malignancy. Lasers Surg. Med., 35: 96–103. doi: 10.1002/lsm.20079

Author Information

  1. 1

    Beckman Laser Institute, University of California, Irvine, California 92612

  2. 2

    University of California, Irvine, California 92612

  3. 3

    University of California, Los Angeles, California 90095

*Beckman Laser Institute, 1002 Health Sciences Road East, University of California, Irvine, CA 92612.

Publication History

  1. Issue published online: 20 AUG 2004
  2. Article first published online: 20 AUG 2004
  3. Manuscript Accepted: 25 APR 2004

Funded by

  • CPRF. Grant Numbers: 27722, 30003
  • CCRP. Grant Number: 00-01391V-20235
  • NIH. Grant Numbers: RO21 CA8752701, EB-00293 CA91717
  • TRDRP. Grant Number: 71T-0192
  • NIH (LAMMP). Grant Number: RR01192
  • DOE. Grant Number: DE903-91ER 61227
  • NSF. Grant Number: BES-86924
  • PMUSA. Grant Number: 32598
  • University of California
  • Irvine Chao Family Comprehensive Cancer Center

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Keywords:

  • carcinoma;
  • non-invasive imaging;
  • leukoplakia;
  • non-invasive diagnostics;
  • oral diagnosis

Abstract

Background and Objective

Current techniques for oral diagnosis require surgical biopsy of lesions, and may fail to detect early malignant change. Non-invasive, sensitive tools providing early detection of oral cancer and a better understanding of malignant change are needed. These studies evaluated in vivo multiphoton excited fluorescence (MPM) techniques to (1) map epithelial and subepithelial changes through out oral carcinogenesis and (2) serve as an effective diagnostic modality.

Study Design/Materials and Methods

In the hamster model (n = 70), epithelial and subepithelial change was imaged in vivo throughout carcinogenesis. MPM- and histopathology-based diagnoses on a scale of 0 (healthy)–6 (squamous cell carcinoma [s.c.c.]) were scored by two pre-standardized investigators.

Results

Collagen matrix and fibers, cellular infiltrates, blood vessels, and microtumors were clearly visible. MPM agreed with the histopathology for 88.6% of diagnoses.

Conclusions

In vivo MPM images provide (1) high resolution information on specific components of the carcinogenesis process (2) an excellent basis for oral diagnostics. Lasers Surg. Med. 35:96–103, 2004. © 2004 Wiley-Liss, Inc.

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