In vivo multiphoton fluorescence imaging: A novel approach to oral malignancy
Article first published online: 20 AUG 2004
Copyright © 2004 Wiley-Liss, Inc.
Lasers in Surgery and Medicine
Volume 35, Issue 2, pages 96–103, August 2004
How to Cite
Wilder-Smith, P., Osann, K., Hanna, N., Abbadi, N. E., Brenner, M., Messadi, D. and Krasieva, T. (2004), In vivo multiphoton fluorescence imaging: A novel approach to oral malignancy. Lasers Surg. Med., 35: 96–103. doi: 10.1002/lsm.20079
- Issue published online: 20 AUG 2004
- Article first published online: 20 AUG 2004
- Manuscript Accepted: 25 APR 2004
- CPRF. Grant Numbers: 27722, 30003
- CCRP. Grant Number: 00-01391V-20235
- NIH. Grant Numbers: RO21 CA8752701, EB-00293 CA91717
- TRDRP. Grant Number: 71T-0192
- NIH (LAMMP). Grant Number: RR01192
- DOE. Grant Number: DE903-91ER 61227
- NSF. Grant Number: BES-86924
- PMUSA. Grant Number: 32598
- University of California
- Irvine Chao Family Comprehensive Cancer Center
- non-invasive imaging;
- non-invasive diagnostics;
- oral diagnosis
Background and Objective
Current techniques for oral diagnosis require surgical biopsy of lesions, and may fail to detect early malignant change. Non-invasive, sensitive tools providing early detection of oral cancer and a better understanding of malignant change are needed. These studies evaluated in vivo multiphoton excited fluorescence (MPM) techniques to (1) map epithelial and subepithelial changes through out oral carcinogenesis and (2) serve as an effective diagnostic modality.
Study Design/Materials and Methods
In the hamster model (n = 70), epithelial and subepithelial change was imaged in vivo throughout carcinogenesis. MPM- and histopathology-based diagnoses on a scale of 0 (healthy)–6 (squamous cell carcinoma [s.c.c.]) were scored by two pre-standardized investigators.
Collagen matrix and fibers, cellular infiltrates, blood vessels, and microtumors were clearly visible. MPM agreed with the histopathology for 88.6% of diagnoses.
In vivo MPM images provide (1) high resolution information on specific components of the carcinogenesis process (2) an excellent basis for oral diagnostics. Lasers Surg. Med. 35:96–103, 2004. © 2004 Wiley-Liss, Inc.