Cell nanosurgery using ultrashort (femtosecond) laser pulses: Applications to membrane surgery and cell isolation

Authors

  • Vikram Kohli,

    1. Department of Electrical and Computer Engineering, Ultrafast Photonics and Nano-Optics Laboratory, University of Alberta, Edmonton, Alberta T6G 2V4, Canada
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  • Abdulhakem Y. Elezzabi,

    Corresponding author
    1. Department of Electrical and Computer Engineering, Ultrafast Photonics and Nano-Optics Laboratory, University of Alberta, Edmonton, Alberta T6G 2V4, Canada
    • Associate Professor, & Canada Research Chair, Department of Electrical and Computer Engineering, Ultrafast Photonics and Nano-Optics Laboratory, University of Alberta, Edmonton, Alberta T6G 2V4, Canada.
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  • Jason P. Acker

    1. Department of Electrical and Computer Engineering, Ultrafast Photonics and Nano-Optics Laboratory, University of Alberta, Edmonton, Alberta T6G 2V4, Canada
    2. Department of Laboratory Medicine and Pathology, University of Alberta, Canadian Blood Services, Edmonton, Alberta T6G 2R8, Canada
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Abstract

Membrane surgery and nanosurgical cell isolation using high-intensity femtosecond laser pulses is reported. We demonstrate the applicability of using ultrashort (femtosecond) laser pulses for performing surgery on live mammalian cells. When sub-10 femtosecond pulses were focused onto the cell, precise sub-micron surgical cuts were made on the biological membrane. Traversing the cells relative to the focused laser spot, we report on localized nanosurgical ablation of focal adhesion adjoining epithelial cells. In each study, the surgery was conducted without morphologically compromising the cells. © 2005 Wiley-Liss, Inc.

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