Raman spectroscopy for optical diagnosis in the larynx: Preliminary findings
Article first published online: 26 AUG 2005
Copyright © 2005 Wiley-Liss, Inc.
Lasers in Surgery and Medicine
Volume 37, Issue 3, pages 192–200, September 2005
How to Cite
Lau, D. P., Huang, Z., Lui, H., Anderson, D. W., Berean, K., Morrison, M. D., Shen, L. and Zeng, H. (2005), Raman spectroscopy for optical diagnosis in the larynx: Preliminary findings. Lasers Surg. Med., 37: 192–200. doi: 10.1002/lsm.20226
- Issue published online: 20 SEP 2005
- Article first published online: 26 AUG 2005
- Manuscript Accepted: 14 JUL 2005
- National Cancer Institute of Canada
- Canadian Cancer Society
- Canadian Dermatology Foundation
- Head and Neck Cancer Foundation of Toronto
- National Medical Research Council of Singapore
- Raman spectroscopy;
- non-invasive diagnosis
Background and Objectives
Raman spectroscopy (RS) provides information about molecular structure and is a potential tool for non-invasive tissue diagnosis. To determine if Raman spectra could be obtained rapidly from laryngeal tissue in vitro, and compare Raman spectra from normal, benign, and cancerous laryngeal tissue.
Study Design/Materials and Methods
Forty-seven laryngeal specimens were studied using RS with signal acquisition times (SAT) between 1 and 30 second(s). Multivariate analysis was used to determine the diagnostic ability of RS compared to standard histology (n = 18, 13, and 16 respectively for normal tissue, carcinoma, and squamous papilloma).
Good quality spectra were obtained with 5-second SAT. Spectral peak analysis showed prediction sensitivities of 89%, 69%, and 88%, and specificities of 86%, 94%, and 94% for normal tissue, carcinoma, and papilloma.
In the larynx, spectral differences appear to exist between normal tissue, carcinoma, and papilloma. The ability to obtain spectra rapidly supports potential for future in vivo studies. © 2005 Wiley-Liss, Inc.