Effects of femtosecond laser irradiation on osseous tissues
Article first published online: 20 FEB 2007
Copyright © 2007 Wiley-Liss, Inc.
Lasers in Surgery and Medicine
Volume 39, Issue 3, pages 273–285, March 2007
How to Cite
Girard, B., Yu, D., Armstrong, M.R., Wilson, B.C., Clokie, C.M.L. and Miller, R.J. D. (2007), Effects of femtosecond laser irradiation on osseous tissues. Lasers Surg. Med., 39: 273–285. doi: 10.1002/lsm.20466
- Issue published online: 7 MAR 2007
- Article first published online: 20 FEB 2007
- Manuscript Accepted: 25 OCT 2006
- Photonics Research of Ontario
- osseous tissue;
- laser ablation;
Background and Objective
Few studies have investigated femtosecond (fs) lasers for cutting bone tissue.
Study Design/Materials and Methods
A 775 nm, 1 kHz, 200 femtosecond, up to 400 µJ laser system was used to irradiate in vitro calcified cortical bone samples and bone tissue culture samples.
The ablation threshold in cortical bone was 0.69±0.08 J/cm2 at 775 nm and 0.19±0.05 J/cm2 at 387 nm. Plasma shielding experiments determined that the ablation plume and the plasma significantly affect material removal at high repetition rates and appear to generate thermal transients in calcified tissue. Confocal analysis revealed intact enzymatic activity on the surface of cells immediately adjacent to cells removed by fs laser irradiation.
These experiments demonstrate that fs lasers used for bone tissue cutting do not appear to generate significant temperature transients to inactivate proteins and that cellular membrane integrity is disrupted for only a few cell layers. Lasers Surg. Med. 39:273–285, 2007. © 2007 Wiley-Liss, Inc.