The anti-inflammatory mechanism of 635 nm light-emitting-diode irradiation compared with existing COX inhibitors
Article first published online: 14 SEP 2007
Copyright © 2007 Wiley-Liss, Inc.
Lasers in Surgery and Medicine
Volume 39, Issue 7, pages 614–621, August 2007
How to Cite
Lim, W., Lee, S., Kim, I., Chung, M., Kim, M., Lim, H., Park, J., Kim, O. and Choi, H. (2007), The anti-inflammatory mechanism of 635 nm light-emitting-diode irradiation compared with existing COX inhibitors. Lasers Surg. Med., 39: 614–621. doi: 10.1002/lsm.20533
- Issue published online: 14 SEP 2007
- Article first published online: 14 SEP 2007
- Manuscript Accepted: 18 JUL 2007
- reactive oxygen species;
- arachidonic acid
Background and Objectives
Inhibition of cyclooxygenase (COX) and prostaglandin E2 (PGE2) protects cells against cell injury in specific pathophysiological situations: inflammation and oxidative stress. Although the anti-inflammatory effects have been reported in clinical fields for specific wavelength irradiation during wound healing, the physiological mechanism has not been clarified yet. The aim of the present study is to investigate the anti-inflammatory mechanism of 635 nm light-emitting-diode (LED) irradiation compared with existing COX inhibitors.
Study Design/Materials and Methods
The present study investigated anti-inflammatory effects of 635 nm irradiation on PGE2 release, COX and phospholipase A2 (PLA2) expression, and reactive oxygen species (ROS) dissociation in arachidonic acid (AA)-treated human gingival fibroblast (hGF). These results were compared with their existing COX inhibitors: indomethacin and ibuprofen. The PGE2 release was measured by enzyme immunoassay, the COX expression was measured by western blot and reverse transcriptase polymerase chain reaction (RT-PCR), and ROS level was measured by flow cytometry, laser scanning confocal microscope and RT-PCR.
Results showed that 635 nm irradiation and existing COX inhibitors inhibit expression of COX and PGE2 release. Unlike indomethacin and ibuprofen, 635 nm irradiation leads to a decrease of ROS levels and mRNA expression of cytosolic phospholipase A2 (cPLA2) and secretary phospholipase A2 (sPLA2).
Taken together, 635 nm irradiation, unlike indomethacin and ibuprofen, can directly dissociate the ROS. This inhibits cPLA2, sPLA2, and COX expression, and results in the inhibition of PGE2 release. Thus, we suggest that 635 nm irradiation inhibits PGE2 synthesis like COX inhibitor and appears to be useful as an anti-inflammatory tool. Lesers Surg. Med. 39:614–621, 2007. © 2007 Wiley-Liss, Inc.