In vivo real time monitoring of vasoconstriction and vasodilation by a combined diffuse reflectance spectroscopy and Doppler optical coherence tomography approach

Authors

  • A. Douplik,

    Corresponding author
    1. Ontario Cancer Institute/Princess Margaret Hospital/University Health Network, Toronto, Canada
    2. Xillix Ltd, Toronto, Canada
    3. Erlangen Graduate School in Advanced Optical Technologies (SAOT), Friedrich-Alexander Universität Erlangen-Nürnberg, Erlangen, Germany
    • Erlangen Graduate School in Advanced Optical Technologies (SAOT), Friedrich-Alexander Universität Erlangen-Nürnberg, Paul-Gordan-Straße 6, 91052 Erlangen, Germany.
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  • D. Morofke,

    1. Ontario Cancer Institute/Princess Margaret Hospital/University Health Network, Toronto, Canada
    2. Department of Engineering Science, University of Oxford, Oxford, UK
    3. Department of Electrical and Computer Engineering, Ryerson University, Toronto, Canada
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  • S. Chiu,

    1. Department of Medical Biophysics, University of Toronto, Toronto, Canada
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  • V. Bouchelev,

    1. Ontario Cancer Institute/Princess Margaret Hospital/University Health Network, Toronto, Canada
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  • L. Mao,

    1. Ontario Cancer Institute/Princess Margaret Hospital/University Health Network, Toronto, Canada
    2. Institute for Microstructural Sciences, National Research Council Canada, Ottawa, Canada
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  • V.X.D. Yang,

    1. Ontario Cancer Institute/Princess Margaret Hospital/University Health Network, Toronto, Canada
    2. Department of Electrical and Computer Engineering, Ryerson University, Toronto, Canada
    3. Imaging Research, Sunnybrook Health Science Centre, Toronto, Canada
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  • A. Vitkin

    1. Ontario Cancer Institute/Princess Margaret Hospital/University Health Network, Toronto, Canada
    2. Department of Medical Biophysics, University of Toronto, Toronto, Canada
    3. Department of Radiation Oncology, University of Toronto, Toronto, Canada
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Abstract

Background and Objectives

A combined diffuse reflectance (DR) spectroscopy and doppler optical coherence tomography (DOCT) approach may offer a powerful means for assessment of tissue function, and potentially provide a way for earlier cancer detection through non-invasive local blood supply measurements. The goal of the study was to compare a DR-derived blood-content-related index to a measure of local blood supply flow as furnished by DOCT during manipulations with blood circulation (vasoconstriction and vasodilation), investigate similarities and differences, complementarity of techniques, and then applying these results to the underlying biology.

Study Design/Materials and Methods

Simultaneous DR–DOCT measurements of local blood supply were conducted during drug and mechanically-induced vasoconstriction and vasodilatation on an externalized intact rat gut in vivo. A simple heuristic metric, termed Blood Supply Index was derived from the spectroscopic DR data. This metric variance due to mechanical and pharmacological manipulation of the local blood supply was recorded, and compared with that of two DOCT-derived metrics, namely normalized blood velocity (vrel) and blood vessel diameter (D).

Summary and Conclusions

During vasoconstriction and vasodilatation, the local blood response was successfully visualized by both DOCT and DR metrics and a reproducible correlation was found between these two measurements. A combined DR-DOCT approach may evolve into a technologically-viable method for cross-validation of the derived haemodynamic metrics, yielding a more reliable functional tissue assessment tool for accurate cancer diagnosis and staging. Lesers Surg. Med. 40:323–331, 2008. © 2008 Wiley-Liss, Inc.

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