SEARCH

SEARCH BY CITATION

Keywords:

  • low level laser therapy;
  • TNF-α;
  • cAMP;
  • NF-κB;
  • airway inflammation;
  • bronchi;
  • rat

Abstract

Background and Objectives

It is unknown if the decreased ability to relax airways smooth muscles in asthma and other inflammatory disorders, such as acute respiratory distress syndrome (ARDS), can be influenced by low level laser therapy (LLLT) irradiation. In this context, the present work was developed in order to investigate if LLLT could reduce dysfunction in inflamed bronchi smooth muscles (BSM) in rats.

Study Design/Materials and Methods

A controlled ex vivo study was developed where bronchi from Wistar rat were dissected and mounted in an organ bath apparatus with or without a TNF-α.

Results

LLLT administered perpendicularly to a point in the middle of the dissected bronchi with a wavelength of 655 nm and a dose of 2.6 J/cm2, partially decreased BSM hyperreactivity to cholinergic agonist, restored BSM relaxation to isoproterenol and reduced the TNF-α mRNA expression. An NF-κB antagonist (BMS205820) blocked the LLLT effect on dysfunction in inflamed BSM.

Conclusion

The results obtained in this work indicate that the LLLT effect on alterations in responsiveness of airway smooth muscles observed in TNF-α-induced experimental acute lung inflammation seems to be dependent of NF-κB activation. Lasers Surg. Med. 41:68–74, 2009. © 2008 Wiley-Liss, Inc.