Basic Science Report
In vivo characterization of human pigmented lesions by degree of linear polarization image maps using incident linearly polarized light
Article first published online: 13 JAN 2010
Copyright © 2010 Wiley-Liss, Inc.
Lasers in Surgery and Medicine
Volume 42, Issue 1, pages 76–85, January 2010
How to Cite
Kim, J., John, R., Wu, P. J., Martini, M. C. and Walsh, J. T. (2010), In vivo characterization of human pigmented lesions by degree of linear polarization image maps using incident linearly polarized light. Lasers Surg. Med., 42: 76–85. doi: 10.1002/lsm.20866
- Issue published online: 13 JAN 2010
- Article first published online: 13 JAN 2010
- Manuscript Accepted: 1 OCT 2009
- Stokes polarimetry imaging;
- degree of linear polarization;
- dysplastic nevus;
- skin cancer;
- incident polarization angle
Background and Objective
Melanoma is the most serious form of skin cancer and often appears as an evolving multicolored skin growth. It is well documented that pre-existing atypical or dysplastic nevi can evolve into a melanoma. The development of an in vivo imaging system to characterize benign and malignant nevi has been emphasized to aid in early detection of melanoma. The goal of this study is to utilize a novel Stokes polarimetry imaging (SPI) system for the characterization of pigmented lesions, and to evaluate the SPI system in comparison to dermoscopy and histology images.
Study Design/Materials and Methods
Linearly polarized light with varying incident polarization angles (IPA) illuminated various types of pigmented lesions. The melanocytic nesting patterns of pigmented lesions were characterized by constructing the degree-of-linear-polarization (DOLP) image map with comparison to dermoscopy and histology. The incident polarized light was filtered by visible filters for spectral imaging and incident deeply penetrating red light was used to correlate the SPI image with histopathological examination.
The DOLP images with varying IPA at different visible wavelengths were used to characterize various kinds of pigmented lesions by showing subsurface melanocytic nesting distribution as well as morphological information with better resolution and contrast. In correlation with dermoscopy and histology, various defining features such as compound, junctional, lentiginous, reticular, globular patterns of melanocytic nests were identified.
When imaging pigmented melanocytic lesions, the SPI system with varying IPA at the red light wavelength can better define the melanocytic nesting patterns in both the dermal epidermal junction and the dermis. The SPI system has the potential to be an effective in vivo method of detecting pre-malignant nevi and melanoma. Lasers Surg. Med. 42:76–85, 2010. © 2010 Wiley-Liss, Inc.