Photodynamic therapy for methicillin-resistant Staphylococcus aureus infection in a mouse skin abrasion model
Article first published online: 13 JAN 2010
Copyright © 2010 Wiley-Liss, Inc.
Lasers in Surgery and Medicine
Volume 42, Issue 1, pages 38–44, January 2010
How to Cite
Dai, T., Tegos, G. P., Zhiyentayev, T., Mylonakis, E. and Hamblin, M. R. (2010), Photodynamic therapy for methicillin-resistant Staphylococcus aureus infection in a mouse skin abrasion model. Lasers Surg. Med., 42: 38–44. doi: 10.1002/lsm.20887
- Issue published online: 13 JAN 2010
- Article first published online: 13 JAN 2010
- Manuscript Accepted: 17 NOV 2009
- NIH. Grant Number: AI050875
- DOD/AFOSR. Grant Number: FA9550-04-1-0079
- photodynamic therapy;
- wound infection;
- skin abrasion;
- mouse model;
- bioluminescence imaging;
- fluorescence imaging
Background and Objective
Methicillin-resistant Staphylococcus aureus (MRSA) skin infections are now known to be a common and important problem in the Unites States. The objective of this study was to investigate the efficacy of photodynamic therapy (PDT) for the treatment of MRSA infection in skin abrasion wounds using a mouse model.
Study Design/Materials and Methods
A mouse model of skin abrasion wound infected with MRSA was developed. Bioluminescent strain of MRSA, a derivative of ATCC 33591, was used to allow the real-time monitoring of the extent of infection in mouse wounds. PDT was performed with the combination of a polyethylenimine (PEI)–ce6 photosensitizer (PS) and non-coherent red light. In vivo fluorescence imaging was carried out to evaluate the effect of photobleaching of PS during PDT.
In vivo fluorescence imaging of conjugate PEI–ce6 applied in mice indicated the photobleaching effect of the PS during PDT. PDT induced on average 2.7 log10 of inactivation of MRSA as judged by loss of bioluminescence in mouse skin abrasion wounds and accelerated the wound healing on average by 8.6 days in comparison to the untreated infected wounds. Photobleaching of PS in the wound was overcome by adding the PS solution in aliquots.
PDT may represent an alternative approach for the treatment of MRSA skin infections. Lasers Surg. Med. 42:38–44, 2010. © 2010 Wiley-Liss, Inc.