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Article first published online: 25 JAN 2012
Copyright © 2011 American Association for the Study of Liver Diseases
Volume 18, Issue 2, pages 226–237, February 2012
How to Cite
Gridelli, B., Vizzini, G., Pietrosi, G., Luca, A., Spada, M., Gruttadauria, S., Cintorino, D., Amico, G., Chinnici, C., Miki, T., Schmelzer, E., Conaldi, P. G., Triolo, F. and Gerlach, J. C. (2012), Efficient human fetal liver cell isolation protocol based on vascular perfusion for liver cell–based therapy and case report on cell transplantation. Liver Transpl, 18: 226–237. doi: 10.1002/lt.22322
Jörg C. Gerlach and Bruno Gridelli designed and coordinated the program, developed the methods for cell isolation and characterization, and participated in writing this article. Giovanni Vizzini and Bruno Gridelli designed and coordinated the clinical work and performed the clinical application. Giada Pietrosi coordinated the study, managed the tissue procurement, and performed the medical care and analytics for the clinical application. Angelo Luca oversaw the cell application and patient monitoring. Marco Spada, Salvatore Gruttadauria, Davide Cintorino, Giandomenico Amico, and Cinzia Chinnici performed the cell isolations. Giandomenico Amico performed the cell characterization and data analysis. Cinzia Chinnici performed the cell characterization. Toshio Miki provided training for the tissue cannulation and cell isolation and performed the literature analysis. Eva Schmelzer performed the literature analysis and the conventional isolations. Pier Giulio Conaldi coordinated the donor screening and the quality control of the cell preparation. Fabio Triolo designed and coordinated the experimental work and cell production and participated in writing this article. All the authors analyzed the data and provided useful suggestions and discussions.
This work was sponsored by a grant from the University of Pittsburgh Medical Center. The development of the good manufacturing practice cell procurement clean room area and the associated quality management system was supported by a grant awarded to the Mediterranean Institute for Transplantation and Advanced Specialized Therapies by the Italian Ministry for Innovation and Technologies and the Region of Sicily.
- Issue published online: 25 JAN 2012
- Article first published online: 25 JAN 2012
- Accepted manuscript online: 27 OCT 2011 07:25AM EST
- Manuscript Accepted: 25 SEP 2011
- Manuscript Received: 14 MAR 2011
- University of Pittsburgh Medical Center
- Mediterranean Institute for Transplantation and Advanced Specialized Therapies by the Italian Ministry for Innovation and Technologies and the Region of Sicily
- 30Autologous human stem cells and nuclear transfer—scientific and ethical issues. L'Osservatore Romano 2001: 6-8., .
- 58Mesenchymal stem cells in human second-trimester bone marrow, liver, lung, and spleen exhibit a similar immunophenotype but a heterogeneous multilineage differentiation potential. Haematologica 2003; 88: 845-852., , , , , , et al.