The authors have no financial disclosures or any other conflicts of interest to disclose.
Efficacy of liver graft washout as a function of the perfusate, pressure, and temperature
Article first published online: 8 JUL 2013
© 2013 American Association for the Study of Liver Diseases
Volume 19, Issue 8, pages 843–851, August 2013
How to Cite
Post, I. C. J. H., Dirkes, M. C., Heger, M., Verheij, J., de Bruin, K. M., de Korte, D., Bennink, R. J. and van Gulik, T. M. (2013), Efficacy of liver graft washout as a function of the perfusate, pressure, and temperature. Liver Transpl, 19: 843–851. doi: 10.1002/lt.23670
- Issue published online: 29 JUL 2013
- Article first published online: 8 JUL 2013
- Accepted manuscript online: 21 MAY 2013 03:54AM EST
- Manuscript Accepted: 14 APR 2013
- Manuscript Received: 17 NOV 2012
Donor graft washout can be impaired by colloids in organ preservation solutions that increase the viscosity and agglutinative propensity of red blood cells (RBCs) and potentially decrease organ function. The colloid-induced agglutinative effects on RBCs and RBC retention after liver washout with Ringer's lactate (RL), histidine tryptophan ketoglutarate solution, University of Wisconsin solution, and Polysol were determined as a function of the washout pressure (15 or 100 mm Hg) and temperature (4 or 37°C) in a rat liver washout model with 99mTc-pertechnetate–labeled RBCs. Colloids (polyethylene glycol in Polysol and hydroxyethyl starch in University of Wisconsin) induced RBC agglutination, regardless of the solution's composition. RL was associated with the lowest degree of 99mTc-pertechnetate–labeled RBC retention after simultaneous arterial and portal washout at 37°C and 100 mm Hg. RL washout was also associated with the shortest washout time. A single portal washout with any of the solutions did not result in differences in the degree of RBC retention, regardless of the temperature or pressure. In conclusion, no differences were found in portal washout efficacy between colloidal solutions, histidine tryptophan ketoglutarate, and RL. Simultaneous arterial and portal washout with RL at 37°C and 100 mm Hg resulted in the least RBC retention and the shortest washout time. Liver Transpl 19:843-851, 2013. © 2013 AASLD.