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Biosynthesis and Compositional Regulation of Poly[(3-hydroxybutyrate)-co-(3-hydroxyhexanoate)] in Recombinant Ralstonia eutropha Expressing Mutated Polyhydroxyalkanoate Synthase Genes

  1. Takeharu Tsuge1,*,
  2. Yu Saito1,
  3. Yoshihiro Kikkawa2,
  4. Tomohiro Hiraishi2,
  5. Yoshiharu Doi1,2

Article first published online: 15 MAR 2004

DOI: 10.1002/mabi.200300077

Issue

Macromolecular Bioscience

Macromolecular Bioscience

Special Issue: Highlights from the First IUPAC International Conference on Bio-based Polymers (ICBP 2003) in Saitama, Japan

Volume 4, Issue 3, pages 238–242, March 15, 2004

Additional Information

How to Cite

Tsuge, T., Saito, Y., Kikkawa, Y., Hiraishi, T. and Doi, Y. (2004), Biosynthesis and Compositional Regulation of Poly[(3-hydroxybutyrate)-co-(3-hydroxyhexanoate)] in Recombinant Ralstonia eutropha Expressing Mutated Polyhydroxyalkanoate Synthase Genes. Macromol. Biosci., 4: 238–242. doi: 10.1002/mabi.200300077

Author Information

  1. 1

    Department of Innovative and Engineered Materials, Tokyo Institute of Technology, 4259 Nagatuta, Midori-ku, Yokohama 226-8502, Japan

  2. 2

    Polymer Chemistry Laboratory, RIKEN Institute, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan

*Department of Innovative and Engineered Materials, Tokyo Institute of Technology, 4259 Nagatuta, Midori-ku, Yokohama 226-8502, Japan. Fax: +81 45 924 5426

Publication History

  1. Issue published online: 15 MAR 2004
  2. Article first published online: 15 MAR 2004
  3. Manuscript Received: 29 OCT 2003
  4. Manuscript Accepted: 7 JAN 2003
  5. Manuscript Revised: 6 JAN 2003

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Keywords:

  • bioengineering;
  • biopolymers;
  • compositional regulation;
  • polyhydroxyalkanoate synthase;
  • mutation

Abstract

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Summary: A new strategy for bacterial polyhydroxyalkanoate (PHA) production by recombinant Ralstonia eutropha PHB−4 harboring mutated PHA synthase genes (phaCAc) from Aeromona caviae was investigated. The strain harboring wild-type phaCAc gene produced a PHA copolymer consisting of (R)-3-hydroxybutyrate and (R)-3-hydroxyhexanoate [P(3HB-co-3HHx)] with 3.5 mol-% of 3HHx fraction from soybean oil. When the mutants of phaCAc gene were applied to this production system, 3HHx fraction in copolymers was varied in the range of 0–5.1 mol-%. Thus, the regulation of PHA copolymer compositions has been achieved by the use of mutated PHA synthase genes.

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