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Metabolic Engineering for the Production of Copolyesters Consisting of 3-Hydroxybutyrate and 3-Hydroxyhexanoate by Aeromonas hydrophila

  1. Yuan-Zheng Qiu1,
  2. Shao-Ping Ouyang1,
  3. Zhongyao Shen2,
  4. Qiong Wu1,
  5. Guo-Qiang Chen1,*

Article first published online: 15 MAR 2004

DOI: 10.1002/mabi.200300090

Issue

Macromolecular Bioscience

Macromolecular Bioscience

Special Issue: Highlights from the First IUPAC International Conference on Bio-based Polymers (ICBP 2003) in Saitama, Japan

Volume 4, Issue 3, pages 255–261, March 15, 2004

Additional Information

How to Cite

Qiu, Y.-Z., Ouyang, S.-P., Shen, Z., Wu, Q. and Chen, G.-Q. (2004), Metabolic Engineering for the Production of Copolyesters Consisting of 3-Hydroxybutyrate and 3-Hydroxyhexanoate by Aeromonas hydrophila. Macromol. Biosci., 4: 255–261. doi: 10.1002/mabi.200300090

Author Information

  1. 1

    Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China

  2. 2

    Department of Chemical Engineering, Tsinghua University, Beijing 100084, China

*Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China. Fax: (+86) 10 6278 8784

Publication History

  1. Issue published online: 15 MAR 2004
  2. Article first published online: 15 MAR 2004
  3. Manuscript Accepted: 8 JAN 2004
  4. Manuscript Revised: 24 DEC 2003
  5. Manuscript Received: 6 NOV 2003

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Keywords:

  • Aeromonas hydrophila;
  • biopolymers;
  • metabolic engineering;
  • polyesters;
  • poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)

Abstract

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Summary:Aeromonas hydrophila 4AK4 was able to synthesize copolyesters consisting of 3-hydroxybutyrate (3HB) and about 15 mol-% 3-hydroxyhexanoate (3HHx) (PHBHHx) when grown in long chain fatty acids such as dodecanoate regardless of growth conditions. To regulate the unit fraction in PHBHHx, phbA and phbB genes encoding β-ketothiolase and acetoacetyl-CoA reductase in Ralstonia eutropha, were introduced into A. hydrophila 4AK4. When gluconate was used as cosubstrate of dodecanoate, the recombinant produced PHBHHx containing 3–12 mol-% 3HHx, depending on the gluconate concentration in media. Vitreoscilla hemoglobin gene, vgb, was also introduced into the above recombinant, resulting in improved PHBHHx content from 38 to 48 wt.-% in shake flask study. Fermentor studies also showed that increased gluconate concentration in medium containing dodecanoate promoted the recombinant strain harboring phbA and phbB genes to incorporate more 3HB unit into PHBHHx, resulting in reduced 3HHx fraction. Recombinant A. hydrophila harboring phbA, phbB and vgb genes demonstrated better PHBHHx productivity and higher conversion efficiency from dodecanoate to PHBHHx than those of the recombinant without vgb in fermentation study. Combined with the robust growth property and simple growth requirement, A. hydrophila 4AK4 appeared to be a useful organism for metabolic engineering.

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