Full Paper

Surface Behavior and Molecular Recognition in DNA Microarrays from N,N-Dimethylacrylamide Terpolymers with Activated Esters as Linking Groups

  1. Raffaella Suriano1,
  2. Marinella Levi1,
  3. Giovanna Pirri2,
  4. Francesco Damin2,
  5. Marcella Chiari2,
  6. Stefano Turri1,*

Article first published online: 11 SEP 2006

DOI: 10.1002/mabi.200600088

Issue

Macromolecular Bioscience

Macromolecular Bioscience

Volume 6, Issue 9, pages 719–729, September 15, 2006

Additional Information

How to Cite

Suriano, R., Levi, M., Pirri, G., Damin, F., Chiari, M. and Turri, S. (2006), Surface Behavior and Molecular Recognition in DNA Microarrays from N,N-Dimethylacrylamide Terpolymers with Activated Esters as Linking Groups. Macromol. Biosci., 6: 719–729. doi: 10.1002/mabi.200600088

Author Information

  1. 1

    Dipartimento di Chimica, Materiali e Ingegneria Chimica “Giulio Natta”, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milan, Italy

  2. 2

    Istituto di Chimica del Riconoscimento Molecolare del CNR, Via Bianco 9, 20131 Milan, Italy

*Dipartimento di Chimica, Materiali e Ingegneria Chimica “Giulio Natta”, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milan, Italy.

Publication History

  1. Issue published online: 28 SEP 2006
  2. Article first published online: 11 SEP 2006
  3. Manuscript Accepted: 14 JUL 2006
  4. Manuscript Revised: 12 JUN 2006
  5. Manuscript Received: 11 APR 2006

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (334K)

Keywords:

  • coatings;
  • DNA;
  • microarrays;
  • molecular recognition;
  • surfaces

Abstract

Thumbnail image of graphical abstract

Summary: A series of terpolymers made of DMA, NAS and MAPS were synthesized by free radical copolymerization and used as functional coatings for the fabrication of glass slide DNA microarrays. The surface properties of coated glass slides were investigated through contact angle measurements, ellipsometry and atomic force microscopy. The terpolymer molecular weight showed a moderate effect on surface tension (γs = 56–62 mN · m−1), but no clear effect on polymeric layer thickness (5–8 nm) and roughness. Hybridization experiments with amine-functionalized oligonucleotides gave the best fluorescence intensity results for microarrays coated with intermediate-molecular-weight terpolymers. Finally, an accelerated ageing test of the microarray in a humidity chamber showed a nice relationship between decay curves of contact angle against water and fluorescence intensity.

View Full Article (HTML) Get PDF (334K)