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Uptake Mechanism of Oppositely Charged Fluorescent Nanoparticles in HeLa Cells

Authors

  • Julia Dausend,

    1. Institute of Organic Chemistry III – Macromolecular Chemistry and Organic Materials, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany
    2. Department of Transfusion Medicine, Institute for Clinical Transfusion Medicine and Immunogenetics Ulm, University of Ulm, Helmholtzstraße 10, 89081 Ulm, Germany
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  • Anna Musyanovych,

    1. Institute of Organic Chemistry III – Macromolecular Chemistry and Organic Materials, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany
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  • Martin Dass,

    1. Institute of Organic Chemistry III – Macromolecular Chemistry and Organic Materials, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany
    2. Central Facility for Electron Microscopy, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany
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  • Paul Walther,

    1. Central Facility for Electron Microscopy, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany
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  • Hubert Schrezenmeier,

    1. Department of Transfusion Medicine, Institute for Clinical Transfusion Medicine and Immunogenetics Ulm, University of Ulm, Helmholtzstraße 10, 89081 Ulm, Germany
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  • Katharina Landfester,

    1. Institute of Organic Chemistry III – Macromolecular Chemistry and Organic Materials, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany
    2. Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany
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  • Volker Mailänder

    Corresponding author
    1. Department of Transfusion Medicine, Institute for Clinical Transfusion Medicine and Immunogenetics Ulm, University of Ulm, Helmholtzstraße 10, 89081 Ulm, Germany
    2. Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany
    • Department of Transfusion Medicine, Institute for Clinical Transfusion Medicine and Immunogenetics Ulm, University of Ulm, Helmholtzstraße 10, 89081 Ulm, Germany
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Abstract

The endocytotic mechanisms involved in the uptake of charged polystyrene nanoparticles into HeLa cells were investigated. Uptake experiments were done in the presence or absence of drugs known to inhibit various factors in endocytosis. Independent of the particle charge, endocytosis is highly dependent on dynamin, F-actin, and tyrosine-specific protein kinases, which suggests a dynamin-dependent and lipid raft-dependent mechanism. However, cholesterol depletion did not hinder particle uptake. Regarding positively charged particles, macropinocytosis, the microtubule network, and cyclooxygenases are also involved. The clathrin-dependent pathway plays a minor role.

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