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Layer-by-Layer Buildup of Poly(L-glutamic acid)/Chitosan Film for Biologically Active Coating

Authors

  • Zhijiang Song,

    1. Department of Polymer Materials, Shanghai University, 20 Chengzhong Street, Jiading, Shanghai 201800, China
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  • Jingbo Yin,

    Corresponding author
    1. Department of Polymer Materials, Shanghai University, 20 Chengzhong Street, Jiading, Shanghai 201800, China
    • Department of Polymer Materials, Shanghai University, 20 Chengzhong Street, Jiading, Shanghai 201800, China
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  • Kun Luo,

    1. Department of Polymer Materials, Shanghai University, 20 Chengzhong Street, Jiading, Shanghai 201800, China
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  • Yanzhen Zheng,

    1. Department of Polymer Materials, Shanghai University, 20 Chengzhong Street, Jiading, Shanghai 201800, China
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  • Yan Yang,

    1. Department of Polymer Materials, Shanghai University, 20 Chengzhong Street, Jiading, Shanghai 201800, China
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  • Qiong Li,

    1. Department of Polymer Materials, Shanghai University, 20 Chengzhong Street, Jiading, Shanghai 201800, China
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  • Shifeng Yan,

    1. Department of Polymer Materials, Shanghai University, 20 Chengzhong Street, Jiading, Shanghai 201800, China
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  • Xuesi Chen

    Corresponding author
    1. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, 130022, China
    • State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, 130022, China
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Abstract

A new biocompatible film based on chitosan and poly(L-glutamic acid) (CS/PGA), created by alternate deposition of CS and PGA, was investigated. FT-IR spectroscopy, UV-vis spectroscopy and QCM were used to analyze the build-up process. The growth of CS and PGA deposition are both exponential to the deposition steps at first. After about 9 (CS/PGA) depositions, the exponential to linear transition takes place. QCM measurements combined with UV-vis spectra revealed the increase in the multilayer film growth at different pH (4.4, 5.0 and 5.5). The build-up of the multilayer stops after a few depositions at pH = 6.5. A muscle myoblast cell (C2C12) assay showed that (CS/PGA)n multilayer films obviously promote C2C12 attachment and growth.

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