Full Paper

The Matrix-Binding Domain of Microfibril-Associated Glycoprotein-1 Targets Active Connective Tissue Growth Factor to a Fibroblast-Produced Extracellular Matrix

  1. Justin S. Weinbaum1,*,
  2. Robert T. Tranquillo1,
  3. Robert P. Mecham2

Article first published online: 26 AUG 2010

DOI: 10.1002/mabi.201000121

Issue

Macromolecular Bioscience

Macromolecular Bioscience

Special Issue: Instructive Materials for Functional Tissue Engineering

Volume 10, Issue 11, pages 1338–1344, November 10, 2010

Additional Information

How to Cite

Weinbaum, J. S., Tranquillo, R. T. and Mecham, R. P. (2010), The Matrix-Binding Domain of Microfibril-Associated Glycoprotein-1 Targets Active Connective Tissue Growth Factor to a Fibroblast-Produced Extracellular Matrix. Macromol. Biosci., 10: 1338–1344. doi: 10.1002/mabi.201000121

Author Information

  1. 1

    Department of Biomedical Engineering, University of Minnesota, 7-105 Hasselmo Hall, 312 Church St. SE, Minneapolis, MN 55455, USA

  2. 2

    Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110, USA

*Department of Biomedical Engineering, University of Minnesota, 7-105 Hasselmo Hall, 312 Church St. SE, Minneapolis, MN 55455, USA. Fax: (+1) 612 626 6583

Publication History

  1. Issue published online: 19 OCT 2010
  2. Article first published online: 26 AUG 2010
  3. Manuscript Revised: 14 MAY 2010
  4. Manuscript Received: 15 MAR 2010

Funded by

  • National Institutes of Health. Grant Numbers: T32 HL007873, HL084922, HL083880

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Keywords:

  • biomaterials;
  • extracellular matrix;
  • fusion protein;
  • growth factor;
  • tissue engineering

Abstract

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It is advantageous to use biomaterials in tissue engineering that stimulate extracellular matrix (ECM) production by the cellular component. Connective tissue growth factor (CTGF) stimulates type I collagen (COL1A1) transcription, but is functionally limited as a free molecule. Using a matrix-binding domain (MBD) from microfibril-associated glycoprotein-1, the fusion protein MBD–CTGF was targeted to the ECM and tested for COL1A1 transcriptional activation. MBD–CTGF produced by the ECM-synthesizing fibroblasts, or provided exogenously, localized to the elastic fiber ECM. MBD–CTGF, but not CTGF alone, led to a two-fold enhancement of COL1A1 expression. This study introduces a targeting technology that can be used to elevate collagen transcription in engineered tissues and thereby improve tissue mechanics.

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