Blood Compatibility and Permeability of Heparin-Modified Polysulfone as Potential Membrane for Simultaneous Hemodialysis and LDL Removal

Authors

  • Xiao-Jun Huang,

    1. Biomedical Materials Group, Department Pharmaceutics and Biopharmaceutics, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany
    2. MOE Key Laboratory of Macromolecular Synthesis and Functionalization (Ministry of Education), Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China
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  • Deepak Guduru,

    1. Biomedical Materials Group, Department Pharmaceutics and Biopharmaceutics, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany
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  • Zhi-Kang Xu,

    1. MOE Key Laboratory of Macromolecular Synthesis and Functionalization (Ministry of Education), Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China
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  • Jörg Vienken,

    1. Fresenius Medical Care Deutschland GmbH, BioSciences Department, Bad Homburg 61352, Germany
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  • Thomas Groth

    Corresponding author
    1. Biomedical Materials Group, Department Pharmaceutics and Biopharmaceutics, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany
    • Biomedical Materials Group, Department Pharmaceutics and Biopharmaceutics, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany Fax: +49 345 5527 379.
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Abstract

Heparin was covalently immobilized on PSf membranes to obtain a dialysis membrane with high affinity for LDL. WCA and streaming potential measurements were performed to investigate wettability and surface charge of the membranes. The morphology of the membranes was investigated by SEM. An ELISA was used to measure the adsorption and desorption of LDL on plain and modified PSf. Blood compatibility was studied by measurement of thrombin time, partial thromboplastin time, kallikrein activity and platelet adhesion. It was found that the blood compatibility of the membrane was improved by covalent immobilization of heparin at its surface. However, PSf-Hep membrane showed higher flux recovery after BSA solution filtration, which revealed antifouling property of PSf-Hep membranes.

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