Novel Oligonucleotide Carrier as Scavenger for Reactive Oxygen Species

Authors

  • Yutaka Ikeda,

    1. Graduate School of Pure and Applied Science, and Tsukuba Research Center for Interdisciplinary Materials Science (TIMS), University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8573, Japan
    2. Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8573, Japan
    Search for more papers by this author
  • Rie Suzuki,

    1. Graduate School of Pure and Applied Science, and Tsukuba Research Center for Interdisciplinary Materials Science (TIMS), University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8573, Japan
    Search for more papers by this author
  • Toru Yoshitomi,

    1. Graduate School of Pure and Applied Science, and Tsukuba Research Center for Interdisciplinary Materials Science (TIMS), University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8573, Japan
    Search for more papers by this author
  • Yukio Nagasaki

    Corresponding author
    1. Graduate School of Pure and Applied Science, and Tsukuba Research Center for Interdisciplinary Materials Science (TIMS), University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8573, Japan
    2. Center for Tsukuba Advanced Research Alliance, University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8573, Japan
    3. Satellite Laboratory, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8573, Japan, and Master's School of Medical Sciences, Graduate School of Comprehensive Human Science, University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8573, Japan
    • Graduate School of Pure and Applied Science, University of Tsukuba, 1-1-1 Ten-noudai, Tsukuba, Ibaraki 305-8573, Japan.
    Search for more papers by this author

Abstract

A novel oligonucleotide carrier which can scavenge ROS is described. The synthesized graft polymer is composed of a PEG segment and a TEMPO-containing hydrophobic segment for scavenging ROS. This graft polymer can form a PIC through electrostatic interaction with oligonucleotides such as siRNA. The amount of ROS was monitored by fluorescence measurements using H2DCFDA as a probe, and it was confirmed that the ROS level was effectively suppressed. The cellular uptake of PIC containing the fluorescence-labeled oligonucleotide was evaluated by fluorescence microscopy. Delivered siRNA suppressed the expression of the mRNA. The prepared graft copolymer is thus a promising candidate as a novel oligonucleotide carrier which also reduces ROS damage generated by cationic polymer carriers.

original image

Ancillary