Thermoresponsive Gene Carriers Based on Polyethylenimine-graft-Poly[oligo(ethylene glycol) methacrylate]

Authors

  • Rui Zhang,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
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  • Yang Wang,

    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
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  • Fu-Sheng Du,

    Corresponding author
    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
    • Beijing National Laboratory for Molecular Sciences, Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
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  • Ying-Li Wang,

    1. Department of Molecular Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China
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  • Ying-Xia Tan,

    1. Department of Molecular Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China
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  • Shou-Ping Ji,

    Corresponding author
    1. Department of Molecular Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China
    • Department of Molecular Biology, Beijing Institute of Transfusion Medicine, Beijing 100850, China.
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  • Zi-Chen Li

    Corresponding author
    1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
    • Beijing National Laboratory for Molecular Sciences, Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
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Abstract

A family of thermoresponsive cationic copolymers (TCPs) that contain branched PEI 25 K as the cationic segment and poly(MEO2MA-co-OEGMA475) as the thermosensitive block (TP) is prepared. The DNA binding capability, physicochemical properties, and biological performance of the TCPs are studied. All of these TCPs can condense DNA to form polyplexes with diameters of 150–300 nm and zeta potentials of 7–32 mV at N/P ratios between 12 and 36. The length of TP block is a key factor for shielding the positive surface charge of the polyplexes and protecting them against protein adsorption. TCPs with a higher TP content have a lower cytotoxicity while the best transfection performance is achieved by the TCPs with longest TP length, reaching a level of the intact PEI 25 K in the presence of serum.

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