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Viability of Human Mesenchymal Stem Cells Seeded on Crosslinked Entropy-Elastic Gelatin-Based Hydrogels

  1. Benjamin F. Pierce1,
  2. Erik Pittermann2,
  3. Nan Ma1,2,
  4. Tim Gebauer1,
  5. Axel T. Neffe1,
  6. Magdalena Hölscher2,
  7. Friedrich Jung1,
  8. Andreas Lendlein1,*

Article first published online: 7 FEB 2012

DOI: 10.1002/mabi.201100237

Issue

Macromolecular Bioscience

Macromolecular Bioscience

Volume 12, Issue 3, pages 312–321, March 2012

Additional Information

How to Cite

Pierce, B. F., Pittermann, E., Ma, N., Gebauer, T., Neffe, A. T., Hölscher, M., Jung, F. and Lendlein, A. (2012), Viability of Human Mesenchymal Stem Cells Seeded on Crosslinked Entropy-Elastic Gelatin-Based Hydrogels. Macromol. Biosci., 12: 312–321. doi: 10.1002/mabi.201100237

Author Information

  1. 1

    Center for Biomaterial Development and Berlin-Brandenburg Center for Regenerative Therapies, Institute of Polymer Research, Helmholtz-Zentrum Geesthacht, Kantstr. 55, 14513 Teltow, Germany

  2. 2

    Reference and Translational Center for Cardiac Stem Cell Therapy, Department of Cardiac Surgery, University of Rostock, Schillingallee 69, 18057 Rostock, Germany

*Center for Biomaterial Development and Berlin-Brandenburg Center for Regenerative Therapies, Institute of Polymer Research, Helmholtz-Zentrum Geesthacht, Kantstr. 55, 14513 Teltow, Germany.

Publication History

  1. Issue published online: 5 MAR 2012
  2. Article first published online: 7 FEB 2012
  3. Manuscript Revised: 1 SEP 2011
  4. Manuscript Received: 14 JUN 2011

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Keywords:

  • biomaterials;
  • gelatin;
  • hydrogels;
  • mesenchymal stem cells (MSCs);
  • structure-property relations

Abstract

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Biomimetic polymer network systems with tailorable properties based on biopolymers represent a class of degradable hydrogels that provides sequences for protein adsorption and cell adhesion. Such materials show potential for in vitro MSC proliferation as well as in vivo applications and were obtained by crosslinking different concentrations of gelatin using varying amounts of ethyl lysine diisocyanate in the presence of a surfactant in pH 7.4 PBS solution. Material extracts, which were tested for cytotoxic effects using L929 mouse fibroblasts, were non-toxic. The hydrogels were seeded with human bone marrow-derived MSCs and supported viable MSCs for the incubation time of 9 d. Preadsorption of fibronectin on materials improved this biofunctionality.

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