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PEGylated Click Polypeptides Synthesized by Copper-Free Microwave-Assisted Thermal Click Polymerization for Selective Endotoxin Removal from Protein Solutions

Authors

  • Jinshan Guo,

    1. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, No. 5625 Renmin Str., Changchun 130022, China
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  • Fanbo Meng,

    Corresponding author
    1. The Cardiology Department of China-Japan Union Hospital of Jilin University, No. 126 Xiantai Str., Changchun 130033, China
    • The Cardiology Department of China-Japan Union Hospital of Jilin University, No. 126 Xiantai Str., Changchun 130033, China.
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  • Xiaoyuan Li,

    1. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, No. 5625 Renmin Str., Changchun 130022, China
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  • Mingzhe Wang,

    1. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, No. 5625 Renmin Str., Changchun 130022, China
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  • Yanjuan Wu,

    1. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, No. 5625 Renmin Str., Changchun 130022, China
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  • Xiabin Jing,

    1. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, No. 5625 Renmin Str., Changchun 130022, China
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  • Yubin Huang

    Corresponding author
    1. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, No. 5625 Renmin Str., Changchun 130022, China
    • State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, No. 5625 Renmin Str., Changchun 130022, China.
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Abstract

PEGylated click polypeptides (PEG-CPs) containing α-amino side groups as well as PEG segments are designed for selective endotoxin removal from protein solutions. The PEG-CPs are synthesized via copper-free thermal click copolymerization from aspartic (or glutamic) acid-based dialkyne and diazide monomers (containing free amino side groups) and alkyne-terminated mPEGs or dialkyne-terminated PEGs. Microwave-assisting technology is introduced into thermal click chemistry to improve the reaction efficiency. The monomers and polymers are fully characterized using NMR, XPS, and MALDI-TOF MS. After immobilizing the PEGylated click polypeptides onto polystyrene microspheres, the adsorbents exhibit good endotoxin removal selectivity from BSA solutions.

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