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Keywords:

  • lysozyme;
  • patterning;
  • proteins;
  • site-specific immobilization;
  • surfaces

Abstract

Thumbnail image of graphical abstract

When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001–0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a “superglue” that binds strongly and quickly onto non-fouling coatings. This binding is highly selective towards lysozyme, and excludes synthetic, chemical/physical activation/deactivation (blocking) steps. By using biotinylated lysozyme, such a phase transition quickly creates a perfect biotinylated surface on non-fouling surfaces for avidin binding, showing great potential for the development of low-cost and practical biochips.