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Direct Biomolecule Binding on Nonfouling Surfaces via Newly Discovered Supramolecular Self-Assembly of Lysozyme under Physiological Conditions

Authors

  • Peng Yang

    Corresponding author
    1. Center for Biologically Inspired Materials and Materials Systems (CBIMMS), Department of Biomedical Engineering, Duke University, 27705, NC, USA
    2. Current address: Department of Chemistry and Biotechnology, University of Tokyo, Bunkyo-ku, Tokyo, Japan
    • Center for Biologically Inspired Materials and Materials Systems (CBIMMS), Department of Biomedical Engineering, Duke University, 27705, NC, USA.
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Abstract

When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001–0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a “superglue” that binds strongly and quickly onto non-fouling coatings. This binding is highly selective towards lysozyme, and excludes synthetic, chemical/physical activation/deactivation (blocking) steps. By using biotinylated lysozyme, such a phase transition quickly creates a perfect biotinylated surface on non-fouling surfaces for avidin binding, showing great potential for the development of low-cost and practical biochips.

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