“Click & Seed” Approach to the Biomimetic Modification of Material Surfaces

Authors

  • Vladimír Proks,

    Corresponding author
    1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovsky sq. 2, Prague 6, 16206, Czech Republic
    • Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovsky sq. 2, Prague 6, 16206, Czech Republic.
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  • Josef Jaroš,

    1. Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 5, Brno, 625 00 Czech Republic
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  • Ognen Pop-Georgievski,

    1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovsky sq. 2, Prague 6, 16206, Czech Republic
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  • Jan Kučka,

    1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovsky sq. 2, Prague 6, 16206, Czech Republic
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  • Štěpán Popelka,

    1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovsky sq. 2, Prague 6, 16206, Czech Republic
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  • Petr Dvořák,

    1. Department of Biology, Faculty of Medicine, Masaryk University, Kamenice 5, Brno, 625 00 Czech Republic
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  • Aleš Hampl,

    1. Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Kamenice 5, Brno, 625 00 Czech Republic
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  • František Rypáček

    1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Heyrovsky sq. 2, Prague 6, 16206, Czech Republic
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Abstract

A simple, versatile, protein-repulsive, substrate-independent biomimetic surface modification is presented that is based on the creation of a PEO brush on a polydopamine anchoring layer and its capacity for selective follow-up modifications with various ligands using a copper-catalyzed alkyne-azide cycloaddition reaction. The desired surface concentration of peptide biomimetic ligands can be controlled by adjusting the peptide concentration in the reaction mixture, then measuring the activity of 125I-radiolabeled peptides that are immobilized on the substrates. The performance of the prepared substrates is tested in cell cultures with MEF cells and a human ECC line.

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