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Non-Adhesive Behavior of New Nanostructured PNIPAM Surfaces Towards Specific Neurodegenerative Proteins: Application to Storage and Titration of Tau Proteins

Authors

  • Tjasa Vrlinic,

    1. Institut des Molécules et Matériaux du Mans, Département Polymères, Colloïdes et Interfaces, LUNAM Université, UMR Université du Maine – CNRS no. 6283, Avenue Olivier Messiaen, 72085 Le Mans, France
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  • Dominique Debarnot,

    1. Institut des Molécules et Matériaux du Mans, Département Polymères, Colloïdes et Interfaces, LUNAM Université, UMR Université du Maine – CNRS no. 6283, Avenue Olivier Messiaen, 72085 Le Mans, France
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  • Gilbert Legeay,

    1. CTTM, 20 rue Thalès de Milet 72000 Le Mans, France
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  • Arnaud Coudreuse,

    1. CTTM, 20 rue Thalès de Milet 72000 Le Mans, France
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  • Benaissa El Moualij,

    1. Institute of Human Histology-CRPP, Université de Liège, 1, avenue de l'hôpital, Sart Tilman, B-4000 Liège, Belgium
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  • Willy Zorzi,

    1. Institute of Human Histology-CRPP, Université de Liège, 1, avenue de l'hôpital, Sart Tilman, B-4000 Liège, Belgium
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  • Armand Perret-Liaudet,

    1. Center Mémoire de Ressources et Recherche, Service de Neurobiologie, Groupement Hospitalier Est du CHU, 56 bd Pinel, 69677 Bron cedex, France
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  • Isabelle Quadrio,

    1. Center Mémoire de Ressources et Recherche, Service de Neurobiologie, Groupement Hospitalier Est du CHU, 56 bd Pinel, 69677 Bron cedex, France
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  • Miran Mozetic,

    1. Jozef Stefan Institute, Jamova cesta 39, 1000 Ljubljana, Slovenia
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  • Fabienne Poncin-Epaillard

    Corresponding author
    1. Institut des Molécules et Matériaux du Mans, Département Polymères, Colloïdes et Interfaces, LUNAM Université, UMR Université du Maine – CNRS no. 6283, Avenue Olivier Messiaen, 72085 Le Mans, France
    • Institut des Molécules et Matériaux du Mans, Département Polymères, Colloïdes et Interfaces, LUNAM Université, UMR Université du Maine – CNRS no. 6283, Avenue Olivier Messiaen, 72085 Le Mans, France
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Abstract

New nonfouling tubes are developed and their influence on the adhesion of neuroproteins is studied. The biomarkers are considered as single components (recombinant prion and Tau proteins) or in a solution of native and pathological forms. The samples are stored for 24 h at 4 °C in virgin and treated tubes layered with two different nanostructured coatings based on poly(N-isopropylacrylamide) with either a positive or a neutral charge, and the protein adhesion is monitored. The recombinant protein with a high pI is repelled from the nanostructured surface that has a negative ζ potential, whereas the recombinant protein with the lower pI is attracted. Furthermore, in the case of complex solutions, neutral nanostructured surfaces are able to retain all amyloid biomarkers.

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