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Polyfluorene Nanoparticles Coated with Folate-Functionalized Triblock Copolymer: Effective Agents for Targeted Cell Imaging

Authors

  • Li Liu,

    1. Department of Pharmaceutics and Biopharmacy, Philipps-University Marburg, Ketzerbach 63, 35032 Marburg, Germany
    2. School of Pharmacy, Shanghai Jiao Tong University, Dongchuan Road 800, 200240 Shanghai, China
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  • Abhijit Patra,

    1. Makromolekulare Chemie, Bergische University Wuppertal, Gauss-Str. 20, 42119 Wuppertal, Germany
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  • Ullrich Scherf,

    1. Makromolekulare Chemie, Bergische University Wuppertal, Gauss-Str. 20, 42119 Wuppertal, Germany
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  • Thomas Kissel

    Corresponding author
    1. Department of Pharmaceutics and Biopharmacy, Philipps-University Marburg, Ketzerbach 63, 35032 Marburg, Germany
    • Department of Pharmaceutics and Biopharmacy, Philipps-University Marburg, Ketzerbach 63, 35032 Marburg, Germany
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Abstract

An ultra-bright fluorescence probe comprising conjugated polymer nanoparticles is developed for biological imaging. Highly blue fluorescent polyfluorene nanoparticles (PF-NPs) stabilized by sodium dodecylsulfate with an average diameter of 100 nm are prepared by a miniemulsion technique. A folate-conjugated cationic triblock copolymer is employed to coat negatively charged PF-NPs via electrostatic interaction for specific cell imaging of folate receptor over-expressing cancer cells. The coated PF-NPs show a similar size and morphology to the pristine PF-NPs, while the fluorescence intensity is enhanced. Such surface-functionalized PF-NPs are demonstrated to be suitable probes for efficient cell imaging of folate receptor over-expressing KB cells by CLSM and flow cytometry.

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