S. U. Frick and N. Bacher contributed equally to this work.
Functionalized Polystyrene Nanoparticles Trigger Human Dendritic Cell Maturation Resulting in Enhanced CD4+ T Cell Activation
Version of Record online: 5 OCT 2012
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 12, Issue 12, pages 1637–1647, December 2012
How to Cite
Frick, S. U., Bacher, N., Baier, G., Mailänder, V., Landfester, K. and Steinbrink, K. (2012), Functionalized Polystyrene Nanoparticles Trigger Human Dendritic Cell Maturation Resulting in Enhanced CD4+ T Cell Activation. Macromol. Biosci., 12: 1637–1647. doi: 10.1002/mabi.201200223
- Issue online: 4 DEC 2012
- Version of Record online: 5 OCT 2012
- Manuscript Revised: 3 AUG 2012
- Manuscript Received: 26 JUN 2012
- dendritic cell maturation;
- functionalization of polymers;
- polystyrene (PS)
Nanoparticles (NP) represent a promising tool for biomedical applications. Here, sulfonate- and phosphonate-functionalized polystyrene NP are analyzed for their interaction with human monocyte-derived dendritic cells (DC). Immature dendritic cells (iDC) display a higher time- and dose-dependent uptake of functionalized polystyrene NP compared to mature dendritic cells (mDC). Notably, NP induce an enhanced maturation of iDC but not of mDC (upregulation of stimulatory molecules and cytokines). NP-triggered maturation results in a significantly enhanced T cell stimulatory capacity (increased CD4+ T cell proliferation and IFN-γ production), indicating a shift to a pronounced Th1 response. Immunomodulatory properties of NP may be a useful strategy for strengthening the efficacy of NP-based approaches in immunotherapy.