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Full Paper
Shrinking Hydrogel-DNA Spots Generates 3D Microdots Arrays
Article first published online: 18 JAN 2013
DOI: 10.1002/mabi.201200370
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Additional Information
How to Cite
Le Goff, G. C., Blum, L. J. and Marquette, C. A. (2013), Shrinking Hydrogel-DNA Spots Generates 3D Microdots Arrays. Macromol. Biosci., 13: 227–233. doi: 10.1002/mabi.201200370
Publication History
- Issue published online: 20 FEB 2013
- Article first published online: 18 JAN 2013
- Manuscript Revised: 23 NOV 2012
- Manuscript Received: 11 OCT 2012
Keywords:
- chemiluminescence;
- colorimetry;
- DNA;
- hydrogels;
- microarray
Abstract
This report describes a straightforward approach for the achievement of sub-100 micrometers size hydrogel dots supporting DNA immobilization. Hydrogel-DNA spots are arrayed and UV-crosslinked on PolyShrink, an innovative polymer material having the remarkable property of isotropically shrinking under high temperature. Curing the microarray enables then spot miniaturization, resulting in 6 µm thick and 60 µm wide hydrogel dots in which oligonucleotides are immobilized in a 3D hydrophilic environment. The probe immobilization within the hydrogel network and its capacity to detect targets specifically and quantitatively is demonstrated using chemiluminescent as well as colorimetric detection techniques. The hydrogel material improves probe accessibility within the spot, leading to an enhanced sensitivity.