These authors contributed equally to this work.
Mimicking Native Extracellular Matrix with Phytic Acid-Crosslinked Protein Nanofibers for Cardiac Tissue Engineering
Article first published online: 18 JAN 2013
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 13, Issue 3, pages 366–375, March 2013
How to Cite
Ravichandran, R., Seitz, V., Reddy Venugopal, J., Sridhar, R., Sundarrajan, S., Mukherjee, S., Wintermantel, E. and Ramakrishna, S. (2013), Mimicking Native Extracellular Matrix with Phytic Acid-Crosslinked Protein Nanofibers for Cardiac Tissue Engineering. Macromol. Biosci., 13: 366–375. doi: 10.1002/mabi.201200391
- Issue published online: 20 MAR 2013
- Article first published online: 18 JAN 2013
- Manuscript Revised: 26 NOV 2012
- Manuscript Received: 29 OCT 2012
- cardiogenic differentiation;
- ischemic myocardium;
- mesenchymal stem cells;
- phytic acid
A functional scaffold fabricated is developed from natural polymers, favoring regeneration of the ischemic myocardium. Hemoglobin/gelatin/fibrinogen (Hb/gel/fib) nanofibers are fabricated by electrospinning and are characterized for morphology, scaffold composition, functional groups and hydrophilicity. It is hypothesized that ex vivo pretreatment of mesenchymal stem cells (MSCs) using 5-azacytidine and such a functional nanofibrous construct having a high oxygen-carrying potential could lead to enhanced cardiomyogenic differentiation of MSCs and result in superior biological and functional effects. The combination of a functional nanofibrous scaffold composed of natural polymers and crosslinked with a natural crosslinking agent, phytic acid, and stem cell biology may prove to be a novel therapeutic device for treatment of myocardial infarction.