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Mimicking Native Extracellular Matrix with Phytic Acid-Crosslinked Protein Nanofibers for Cardiac Tissue Engineering

  1. Rajeswari Ravichandran1,2,†,
  2. Vera Seitz1,3,†,
  3. Jayarama Reddy Venugopal1,*,
  4. Radhakrishnan Sridhar1,2,
  5. Subramanian Sundarrajan1,2,
  6. Shayanti Mukherjee1,
  7. Erich Wintermantel3,
  8. Seeram Ramakrishna1,2

Article first published online: 18 JAN 2013

DOI: 10.1002/mabi.201200391

Issue

Macromolecular Bioscience

Macromolecular Bioscience

Volume 13, Issue 3, pages 366–375, March 2013

Additional Information

How to Cite

Ravichandran, R., Seitz, V., Reddy Venugopal, J., Sridhar, R., Sundarrajan, S., Mukherjee, S., Wintermantel, E. and Ramakrishna, S. (2013), Mimicking Native Extracellular Matrix with Phytic Acid-Crosslinked Protein Nanofibers for Cardiac Tissue Engineering. Macromol. Biosci., 13: 366–375. doi: 10.1002/mabi.201200391

Author Information

  1. 1

    Health care and Energy Materials Laboratory, Nanoscience and Nanotechnology Initiative, National University of Singapore, Faculty of Engineering, 2 Engineering Drive 3, Singapore 117576

  2. 2

    Department of Mechanical Engineering, National University of Singapore, 2 Engineering Drive 3, Singapore 117576

  3. 3

    Institute of Medical and Polymer Engineering, Technische Universität München (TUM), Boltzmannstrasse 1585748 Garching, Germany

  1. †

    These authors contributed equally to this work.

*Health care and Energy Materials Laboratory, Nanoscience and Nanotechnology Initiative, National University of Singapore, Faculty of Engineering, 2 Engineering Drive 3, Singapore 117576

  1. †

    These authors contributed equally to this work.

Publication History

  1. Issue published online: 20 MAR 2013
  2. Article first published online: 18 JAN 2013
  3. Manuscript Revised: 26 NOV 2012
  4. Manuscript Received: 29 OCT 2012

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Keywords:

  • cardiogenic differentiation;
  • crosslinkers;
  • ischemic myocardium;
  • mesenchymal stem cells;
  • phytic acid

Abstract

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A functional scaffold fabricated is developed from natural polymers, favoring regeneration of the ischemic myocardium. Hemoglobin/gelatin/fibrinogen (Hb/gel/fib) nanofibers are fabricated by electrospinning and are characterized for morphology, scaffold composition, functional groups and hydrophilicity. It is hypothesized that ex vivo pretreatment of mesenchymal stem cells (MSCs) using 5-azacytidine and such a functional nanofibrous construct having a high oxygen-carrying potential could lead to enhanced cardiomyogenic differentiation of MSCs and result in superior biological and functional effects. The combination of a functional nanofibrous scaffold composed of natural polymers and crosslinked with a natural crosslinking agent, phytic acid, and stem cell biology may prove to be a novel therapeutic device for treatment of myocardial infarction.

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