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Mastoid Obliteration Using Three-Dimensional Composite Scaffolds Consisting of Polycaprolactone/β-Tricalcium Phosphate/Collagen Nanofibers: An In Vitro and In Vivo Study

Authors

  • Chul Ho Jang,

    Corresponding author
    1. Department of Otolaryngology, Chonnam National University Medical School, Gwangju
    2. Research Center for Resistant Cells, Chosun Medical School, Gwangju
    • Department of Otolaryngology, Chonnam National University Medical School, Gwangju
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  • Yong Beom Cho,

    1. Department of Otolaryngology, Chonnam National University Medical School, Gwangju
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  • Myung Gu Yeo,

    1. Department of Bio-Mechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea
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  • Geun Hyung Kim

    Corresponding author
    1. Department of Bio-Mechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea
    • Department of Bio-Mechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea
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Abstract

Two different composite scaffolds, solid-freeform-fabricated PCL/β-TCP supplemented with and without collagen nanofibers are fabricated. These scaffolds are evaluated whether a combination of collagen nanofibers with PCL/β-TCP can promote osteogenesis in a mastoid obliteration. To assess the effects of the cellular activities of osteoblast-like-cells (MG63), SEM images and MTT assays are conducted. Experimental mastoid obliteration is performed using guinea pigs that are divided group A (PCL/β-TCP/collagen-nanofiber scaffold) and group B (PCL/β-TCP scaffold). The results reveal that PCL/β-TCP/collagen scaffold provide much broader cell attachment sites than PCL/β-TCP scaffold. The µ-CT and fluorescent microscopy results reveal that the acceleration of early new bone formation within the pores and scaffold itself at week 4 post-operation is more effective in group A. In addition, based on the results of the histological and µ-CT at 12 weeks post-surgery, the effective regeneration of bone in the PCL/β-TCP/collagen scaffold is appeared.

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