A Novel 3D Integrated Platform for the High-Resolution Study of Cell Migration Plasticity

Authors

  • Julian Schneider,

    1. Laboratory of Thermodynamics in Emerging Technologies, Institute of Energy Technology, Department of Mechanical and Process Engineering, ETH Zürich, CH-8092 Zürich, Switzerland
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  • Tobias Bachmann,

    1. Laboratory of Thermodynamics in Emerging Technologies, Institute of Energy Technology, Department of Mechanical and Process Engineering, ETH Zürich, CH-8092 Zürich, Switzerland
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  • Davide Franco,

    1. Laboratory of Thermodynamics in Emerging Technologies, Institute of Energy Technology, Department of Mechanical and Process Engineering, ETH Zürich, CH-8092 Zürich, Switzerland
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  • Patrizia Richner,

    1. Laboratory of Thermodynamics in Emerging Technologies, Institute of Energy Technology, Department of Mechanical and Process Engineering, ETH Zürich, CH-8092 Zürich, Switzerland
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  • Patrick Galliker,

    1. Laboratory of Thermodynamics in Emerging Technologies, Institute of Energy Technology, Department of Mechanical and Process Engineering, ETH Zürich, CH-8092 Zürich, Switzerland
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  • Manish K. Tiwari,

    1. Laboratory of Thermodynamics in Emerging Technologies, Institute of Energy Technology, Department of Mechanical and Process Engineering, ETH Zürich, CH-8092 Zürich, Switzerland
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  • Aldo Ferrari,

    Corresponding author
    • Laboratory of Thermodynamics in Emerging Technologies, Institute of Energy Technology, Department of Mechanical and Process Engineering, ETH Zürich, CH-8092 Zürich, Switzerland
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  • Dimos Poulikakos

    Corresponding author
    • Laboratory of Thermodynamics in Emerging Technologies, Institute of Energy Technology, Department of Mechanical and Process Engineering, ETH Zürich, CH-8092 Zürich, Switzerland
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E-mail: aferrari@ethz.ch; dimos.poulikakos@ethz.ch

Abstract

Understanding the mechanisms of interstitial cancer migration is of great scientific and medical interest. Creating 3D platforms, conducive to optical microscopy and mimicking the physical parameters (in plane and out of plane) involved in interstitial migration, is a major step forward in this direction. Here, a novel approach is used to directly print free-form, 3D micropores on basal scaffolds containing microgratings optimized for contact guidance. The platforms so formed are validated by monitoring cancer cell migration and micropore penetration with high-resolution optical microscopy. The shapes, sizes and deformability of the micropores are controllable, paving the way to decipher their role in interstitial migration.mabi201200416-gra-0001

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