Macromol. Biosci. 2/2012
Tomohiro Hiraishi, Koichi Yamashita, Masafumi Sakono, Jun Nakanishi, Liu-Tzea Tan, Kumar Sudesh, Hideki Abe and Mizuo Maeda
Version of Record online: 3 FEB 2012 | DOI: 10.1002/mabi.201290005
Cover: Cell surface display of enzymes as whole-cell catalysts may be a better choice in comparison with typical enzyme catalysts because of advantages like autonomous replication, preventing the toxicity of the expressed proteins, the stabilization of the displayed enzymes due to self-immobilization on the cells, and so on. The present manuscript focuses on functionally active expression of poly[(R)-3-hydroxybutyrate] (PHB) depolymerase on the cell surface of E. coli and its potential use as a biocatalyst for (R)-3-hydroxybutyrate (3HB) monomer production from insoluble PHB. Further details can be found in the article by T. Hiraishi,* K. Yamashita, M. Sakono, J. Nakanishi, L.-T. Tan, K. Sudesh, H. Abe, and M. Maeda* on page 218.