Macromolecular Bioscience

Cover image for Vol. 12 Issue 2

February 2012

Volume 12, Issue 2

Pages 139–273

  1. Cover Picture

    1. Top of page
    2. Cover Picture
    3. Masthead
    4. Contents
    5. Review
    6. Full Papers
    7. Communication
    1. Macromol. Biosci. 2/2012

      Tomohiro Hiraishi, Koichi Yamashita, Masafumi Sakono, Jun Nakanishi, Liu-Tzea Tan, Kumar Sudesh, Hideki Abe and Mizuo Maeda

      Article first published online: 3 FEB 2012 | DOI: 10.1002/mabi.201290005

      Thumbnail image of graphical abstract

      Cover: Cell surface display of enzymes as whole-cell catalysts may be a better choice in comparison with typical enzyme catalysts because of advantages like autonomous replication, preventing the toxicity of the expressed proteins, the stabilization of the displayed enzymes due to self-immobilization on the cells, and so on. The present manuscript focuses on functionally active expression of poly[(R)-3-hydroxybutyrate] (PHB) depolymerase on the cell surface of E. coli and its potential use as a biocatalyst for (R)-3-hydroxybutyrate (3HB) monomer production from insoluble PHB. Further details can be found in the article by T. Hiraishi,* K. Yamashita, M. Sakono, J. Nakanishi, L.-T. Tan, K. Sudesh, H. Abe, and M. Maeda* on page 218.

  2. Masthead

    1. Top of page
    2. Cover Picture
    3. Masthead
    4. Contents
    5. Review
    6. Full Papers
    7. Communication
    1. Macromol. Biosci. 2/2012

      Article first published online: 3 FEB 2012 | DOI: 10.1002/mabi.201290006

  3. Contents

    1. Top of page
    2. Cover Picture
    3. Masthead
    4. Contents
    5. Review
    6. Full Papers
    7. Communication
    1. Macromol. Biosci. 2/2012 (pages 139–143)

      Article first published online: 3 FEB 2012 | DOI: 10.1002/mabi.201290004

  4. Review

    1. Top of page
    2. Cover Picture
    3. Masthead
    4. Contents
    5. Review
    6. Full Papers
    7. Communication
    1. Copolymers: Efficient Carriers for Intelligent Nanoparticulate Drug Targeting and Gene Therapy (pages 144–164)

      Mehrdad Hamidi, Mohammad-Ali Shahbazi and Kobra Rostamizadeh

      Article first published online: 17 OCT 2011 | DOI: 10.1002/mabi.201100193

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      The goal of copolymer-based drug delivery systems is to deploy therapeutics intact to specifically targeted tissue of the body through a medium capable of controlling the therapy's administration by means of either a physiological or chemical trigger. To this end, scientists are examining copolymer micelles, polymersomes and hydrogels.

  5. Full Papers

    1. Top of page
    2. Cover Picture
    3. Masthead
    4. Contents
    5. Review
    6. Full Papers
    7. Communication
    1. Synthesis of Polyester Nanoparticles in Miniemulsion Obtained by Radical Ring-Opening of BMDO and Their Potential as Biodegradable Drug Carriers (pages 165–175)

      Joerg Max Siebert, Daniela Baumann, Anke Zeller, Volker Mailänder and Katharina Landfester

      Article first published online: 15 NOV 2011 | DOI: 10.1002/mabi.201100236

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      The free-radical miniempulsion copolymerization of BMDO with styrene and MMA is investigated; the obtained polyester and polyester/polystyrene nanoparticles show good uptake into cells and no toxicity. Nanoparticles with incorporated paclitaxel as a hydrophobic drug reveal their potential as a degradable drug delivery system.

    2. A Potential Nanomedicine Consisting of Heparin-Loaded Polysaccharide Nanocarriers for the Treatment of Asthma (pages 176–183)

      Felipe A. Oyarzun-Ampuero, Jose Brea, Maria I. Loza, Maria J. Alonso and Dolores Torres

      Article first published online: 23 NOV 2011 | DOI: 10.1002/mabi.201100102

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      Heparin associated with nanoparticles composed of chitosan and cyclodextrins is investigated for a new application as an antiasthmatic drug. The capacity of the nanoparticles to interact with mastocytes and to prevent the degranulation of mast cells confirms the potential interest of this new nanomedicine for the treatment of asthma.

    3. A Hydrophobic Starch Polymer for Nanoparticle-Mediated Delivery of Docetaxel (pages 184–194)

      Prajakta Dandekar, Ratnesh Jain, Thomas Stauner, Brigitta Loretz, Marcus Koch, Gerhard Wenz and Claus-Michael Lehr

      Article first published online: 29 NOV 2011 | DOI: 10.1002/mabi.201100244

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      Docetaxel loading into nanoparticles of propyl starch, a novel hydrophobic starch derivative, is described. The nanoparticles exhibit a high docetaxel encapsulation and the ability to control its release. Cellular cytotoxicity assays indicate safety and efficacy of nanoparticles over non-encapsulated drug at the test concentrations. Uptake investigations indicate an efficient uptake and peri-nuclear localization of nanoparticles by cancer cells.

    4. Natively Unfolded State for Engineering Nanoscale Fibrillar Arrays (pages 195–201)

      Maxim G. Ryadnov and Dmitry I. Cherny

      Article first published online: 6 DEC 2011 | DOI: 10.1002/mabi.201100295

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      High aspect ratio fibrillar nanoscale arrays are generated from an α-helical fiber. The design is inspired by an intermittence effect observed for native β-structured α-synunclein fibrils. The generated nanoarrays extend to microns in length and are composed of periodic nanosized segments separated at uniform distances of unfolded regions.

    5. Characterization of a Hierarchical Network of Hyaluronic Acid/Gelatin Composite for use as a Smart Injectable Biomaterial (pages 202–210)

      Hossein K. Heris, Meysam Rahmat and Luc Mongeau

      Article first published online: 6 DEC 2011 | DOI: 10.1002/mabi.201100335

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      Hybrid hyaluronic acid–gelatin (HA–Ge) hydrogel particles are embedded in a secondary HA network. The microgels are sufficiently stiff, with the amino acid sequences required for cell adhesion. The soft external network of hyaluronic acid can facilitate non-proteolytic cell migration. The microgel system exhibits pH responsiveness. The HA–Ge hierarchical system characteristics are favorable for tissue engineering applications.

    6. Bacterial Adhesion-Resistant Poly(2-hydroxyethyl methacrylate) Derivative for Mammalian Cell Cultures (pages 211–217)

      Eun-Ho Sohn, Jooyeon Ahn, Suk Ho Bhang, Junil Kang, Jeyong Yoon, Byung-Soo Kim and Jong-Chan Lee

      Article first published online: 6 DEC 2011 | DOI: 10.1002/mabi.201100235

      Thumbnail image of graphical abstract

      Novel PHEMA derivatives for mammalian cell culture are synthesized and characterized. Among the polymers, a PHEMA derivative with highest oxygen content (PHEMAAA) shows its ability to promote human dermal fibroblast cell adhesion and proliferation while effectively decreasing bacterial adhesion.

    7. Display of Functionally Active PHB Depolymerase on Escherichia Coli Cell Surface (pages 218–224)

      Tomohiro Hiraishi, Koichi Yamashita, Masafumi Sakono, Jun Nakanishi, Liu-Tzea Tan, Kumar Sudesh, Hideki Abe and Mizuo Maeda

      Article first published online: 17 NOV 2011 | DOI: 10.1002/mabi.201100273

      Thumbnail image of graphical abstract

      The display of PHB depolymerase (PhaZRpiT1) on the surface of E. coli is realized using OprI from P. aeruginosa. The displayed PhaZRpiT1 retained its cleaving ability for soluble substrates as well as its adsorbing ability to PHB surface. The displayed enzyme also retains its inherent ability to degrade PHB, demonstrating its potential use for (R)-3-hydroxybutyrate production from PHB materials.

    8. Poly(amidoamine)-based Dendrimer/siRNA Complexation Studied by Computer Simulations: Effects of pH and Generation on Dendrimer Structure and siRNA Binding (pages 225–240)

      Kostas Karatasos, Paola Posocco, Erik Laurini and Sabrina Pricl

      Article first published online: 6 DEC 2011 | DOI: 10.1002/mabi.201100276

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      Results obtained from molecular dynamics simulations of high-generation PAMAM-based dendrimers having NH3and triethanolamine as cores, forming complexes with a short interfering RNA at different pH values and at physiological ionic strength, are reported. The structural flexibility of the triethanolamine-core dendrimer allows a higher degree of penetration of the siRNA strands within the dendritic structure resulting in the formation of more stable complexes.

    9. Synthesis and Characterization of Novel Biodegradable and Electroactive Hydrogel Based on Aniline Oligomer and Gelatin (pages 241–250)

      Yadong Liu, Jun Hu, Xiuli Zhuang, Peibiao Zhang, Yen Wei, Xianhong Wang and Xuesi Chen

      Article first published online: 25 OCT 2011 | DOI: 10.1002/mabi.201100227

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      Biodegradable electroactive hydrogel (AP-g-GA), aniline pentamer (AP) grafting gelatin (GA), is synthesized. The hydrophobic AP changes the hydrogel's porous structure of the natural GA. With increasing content of AP, the hydrogel gradually forms porous structures, from “honeycomb” to “bamboo raft”. The AP-g-GA polymers can accelerate the proliferation of osteoblasts compared with pure GA and TCPS.

    10. A Multifunctional Nanocarrier Based on Nanogated Mesoporous Silica for Enhanced Tumor-Specific Uptake and Intracellular Delivery (pages 251–259)

      Yaohua Gao, Cuihong Yang, Xue Liu, Rujiang Ma, Deling Kong and Linqi Shi

      Article first published online: 11 NOV 2011 | DOI: 10.1002/mabi.201100208

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      A multifunctional drug carrier based on polypseudorotaxane(PEG/CD)-nanogated mesoporous silica is presented. It combines multifunctionality, i.e., enhanced tumor-specific cellular uptake, intracellular delivery, biocompatibility, and potential in vivo longevity in one nanoparticle and offers therefore a huge potential in targeting tumor therapy.

    11. Assessing the Degradation Profile of Functional Aliphatic Polyesters with Precise Control of the Degradation Products (pages 260–268)

      Anders Höglund, Sofia Målberg and Ann-Christine Albertsson

      Article first published online: 17 NOV 2011 | DOI: 10.1002/mabi.201100288

      Thumbnail image of graphical abstract

      The pre-polymer poly(but-2-ene-1,4-diyl malonate) (PBM) and an array of PBM-based materials are shown to be degradable under physiological conditions in vitro with control over the formed degradation products. The PBM-based materials thus have potential as materials for future biomedical applications.

  6. Communication

    1. Top of page
    2. Cover Picture
    3. Masthead
    4. Contents
    5. Review
    6. Full Papers
    7. Communication
    1. Metallization of a Genetically Engineered Polypeptide (pages 269–273)

      Autumn Carlsen, Seiichiro Higashiya, Natasha I. Topilina, Kathleen A. Dunn, Robert E. Geer, Eric T. Eisenbraun, Alain E. Kaloyeros and John T. Welch

      Article first published online: 6 DEC 2011 | DOI: 10.1002/mabi.201100245

      Thumbnail image of graphical abstract

      Genetically engineered, β-sheet-containing fibrils can be platinated via reductive metallization. Localization of the platinum nanoparticles along the fibril edges is determined by transmission electron microscopy and energy dispersive X-ray data analysis. The platinated fibrils exhibit clear evidence of metallization at the functionalized turn-residues of the fibrillar constructs.

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