Novel Synthesis of HPMA Copolymers Containing Peptide Grafts and Their Self-Assembly Into Hybrid Hydrogels

Authors

  • Kuangshi Wu,

    1. Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
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  • Jiyuan Yang,

    1. Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
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  • Čestmír Koňák,

    1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 16206 Prague 6, Czech Republic
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  • Pavla Kopečková,

    1. Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    2. Department of Bioengineering, University of Utah, Salt Lake City, Utah 84112, USA
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  • Jindřich Kopeček

    Corresponding author
    1. Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, Utah 84112, USA
    2. Department of Bioengineering, University of Utah, Salt Lake City, Utah 84112, USA
    • Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, Utah 84112, USA.
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Abstract

A new pathway for the synthesis of HPMA graft copolymers was developed and their self-assembly into hybrid hydrogels was investigated. Linear water-soluble polyHPMA was chosen as the polymer backbone, whereas coiled-coil forming peptides, covalently attached to the backbone, formed the grafts. Peptides of different chain lengths were chosen for evaluation. The results revealed that graft length greatly affected gelation ability. At least four heptads were needed to facilitate the hybrid system to form hydrogels under the experimental conditions used. The major factors affecting the kinetics of hydrogel self-assembly were concentration and temperature.

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