Disulfide-Containing Hyperbranched Polyethylenimine Derivatives via Click Chemistry for Nonviral Gene Delivery

Authors

  • Xulin Jiang,

    Corresponding author
    1. Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University, Wuhan 430072, China
    • Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University, Wuhan 430072, China. Fax: +86 27 6875 4509.
    Search for more papers by this author
  • Jia Liu,

    1. Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University, Wuhan 430072, China
    Search for more papers by this author
  • Li Xu,

    1. Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University, Wuhan 430072, China
    Search for more papers by this author
  • Renxi Zhuo

    1. Key Laboratory of Biomedical Polymers of Ministry of Education and Department of Chemistry, Wuhan University, Wuhan 430072, China
    Search for more papers by this author

Abstract

Reducible disulfide-containing hyperbranched PEI-SS-HP was synthesized via click chemistry and evaluated as nonviral gene carrier. The structure of the polymers was confirmed by 1H NMR and FTIR. It was shown that the PEI-SS-HP was able to bind plasmid DNA to positively charged nanoparticles. The reduction sensitivity of the PEI-SS-HP was confirmed by gel retardation assay in the presence of DTT mimicking an intracellular reductive environment. In vitro experiments revealed that the reducible PEI-SS-HP not only had much lower cytotoxicity, but also posed superior transfection activity as compared to non-degradable 25-kDa PEI. The results indicate that a reducibly degradable PEI-SS-HP can be a promising gene vector.

original image

Ancillary