Electrospun Composite Mats of Poly[(D,L-lactide)-co-glycolide] and Collagen with High Porosity as Potential Scaffolds for Skin Tissue Engineering

Authors

  • Ye Yang,

    1. Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science & Engineering, Southwest Jiaotong University, Chengdu 610031, China
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  • Xinli Zhu,

    1. Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science & Engineering, Southwest Jiaotong University, Chengdu 610031, China
    2. Research and Development Center for Tissue Engineering, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China
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  • Wenguo Cui,

    1. Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science & Engineering, Southwest Jiaotong University, Chengdu 610031, China
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  • Xiaohong Li,

    Corresponding author
    1. Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science & Engineering, Southwest Jiaotong University, Chengdu 610031, China
    • Key Laboratory of Advanced Technologies of Materials, Ministry of Education, School of Materials Science & Engineering, Southwest Jiaotong University, Chengdu 610031, China. Fax: +86 28 8763 4649
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  • Yan Jin

    Corresponding author
    1. Research and Development Center for Tissue Engineering, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China
    • Research and Development Center for Tissue Engineering, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China. Fax: +86 29 8321 8039
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Abstract

Electrospun composite mats of poly[(D,L-lactide)-co-glycolide] and collagen with high porosities of 85–90% and extended pore sizes of 90–130 µm were prepared to mimic the ECM morphologically and chemically. The existence of collagen molecules on the fiber surface was confirmed, enabling the cells to find enhanced binding sites for their integrin receptors. The mechanical data for the blended fibrous mats indicated that they were sufficiently durable for dermal tissue engineering. Fibroblasts derived from GFP transgenic C57BL/6 mice were used to directly observe cell proliferation, and the inoculation of collagen enhanced cell attachment, proliferation and extracellular matrix secretion, which were found to be dependent on the amount of collagen in the composite scaffold.

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