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Core/Shell Polymer Particles by Semibatch Combined Suspension/Emulsion Polymerizations for Enzyme Immobilization

Authors

  • Marina D. Besteti,

    1. Programa de Engenharia Química/COPPE, Universidade Federal do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, RJ, Brazil
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  • Aline G. Cunha,

    1. Programa de Pós-Graduação em Bioquímica/Instituto de Química, Universidade Federal do Rio de Janeiro, Cidade Universitária, Centro de Tecnologia, Rio de Janeiro, RJ, Brazil
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  • Denise M. G. Freire,

    1. Programa de Pós-Graduação em Bioquímica/Instituto de Química, Universidade Federal do Rio de Janeiro, Cidade Universitária, Centro de Tecnologia, Rio de Janeiro, RJ, Brazil
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  • José Carlos Pinto

    Corresponding author
    1. Programa de Engenharia Química/COPPE, Universidade Federal do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, RJ, Brazil
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Abstract

Semibatch combined suspension–emulsion polymerizations are performed for production of polymer supports to be used for immobilization of lipase B from Candida antarctica (CALB), using styrene, methyl methacrylate, and cardanol as monomers. It is shown that the obtained polymer particles present the characteristic core–shell particle structure, with specific areas and average pore sizes that are comparable to values presented by Accurel MP 1000. It is also shown that the produced particles can be used successfully for immobilization of CALB, leading to immobilization efficiencies and enzyme activities that are better than the ones obtained with Accurel MP 1000.mame201300023-gra-0001

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