Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles

Authors

  • Nemanja Miletić,

    1. Department of Polymer Chemistry, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
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  • Volker Abetz,

    1. GKSS Research Centre Geesthacht GmbH, Institute of Materials Research, Max-Planck-Str. 1, 21502 Geesthacht, Germany
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  • Katrin Ebert,

    1. GKSS Research Centre Geesthacht GmbH, Institute of Materials Research, Max-Planck-Str. 1, 21502 Geesthacht, Germany
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  • Katja Loos

    Corresponding author
    1. Department of Polymer Chemistry, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    • Department of Polymer Chemistry, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. Fax: (+31) 50 3634400.
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Abstract

Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal-B) and on the hydrolytic activity (hydrolysis of p-nitrophenyl acetate) of the immobilized Cal-B was studied. The pH of the buffer solution has no influence on enzyme loading, while immobilized enzyme activity is very dependent on the pH of adsorption. Cal-B immobilized on PS nanoparticles in buffer solution pH 6.8 performed higher hydrolytic activity than crude enzyme powder and Novozyme 435.

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