High-Density Glycosylation of Polymer Membrane Surfaces by Click Chemistry for Carbohydrate–Protein Recognition

Authors

  • Cang Wang,

    1. Department of Chemistry, Zhejiang University, Hangzhou 310027, P. R. China
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  • Jian Wu,

    1. Department of Chemistry, Zhejiang University, Hangzhou 310027, P. R. China
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  • Zhi-Kang Xu

    Corresponding author
    1. MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, P. R. China
    • MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, P. R. China Fax: + 86 571 8795 1773.
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Abstract

To biologically mimic the carbohydrate–protein interactions in artificial systems, one of the challenges is to construct a glycosylated surface with a high glycosyl density to yield a notable ‘glycoside cluster effect’. A novel strategy is presented for high density glycosylation of the surface of a microporous poly(propylene) membrane (MPPM) by click chemistry. It is promising that the surface glycosyl density can be well controlled over a wide range and the maximum value is over 10 µmol · cm−2. The recognition capability of these glycosylated MPPMs to lectins indicates the occurrence of the ‘glycoside cluster effect’ when the glycosyl density on the membrane surface exceeds 0.20 µmol · cm−2.

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