Coordination Polymer Nanobelts as an Effective Sensing Platform for Fluorescence-enhanced Nucleic Acid Detection

Authors

  • Hailong Li,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
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  • Lei Wang,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China
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  • Junfeng Zhai,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China
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  • Yingwei Zhang,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China
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  • Jingqi Tian,

    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China
    2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
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  • Xuping Sun

    Corresponding author
    1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China
    • State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China.
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Abstract

In this communication, the application of coordination polymer nanobelts (CPNs) assembled from H2PtCl6 and 3,3′,5,5′-tetramethylbenzidine (TMB) are explored as an effective fluorescent sensing platform for nucleic acid detection for the first time. The suggested method has a high selectivity down to single-base mismatch. DNA detection is accomplished by the following two steps: (1) CPN binds fluorecent dye-labeled single-stranded DNA (ssDNA) probe via both electrostatic attraction and π-π stacking interactions between unpaired DNA bases and CPN. As a result, the fluorescent dye is brought into close proximity to CPN and substantial fluorescence quenching occurs due to photoinduced electron transfer from the nitrogen atom in CPN to the excited fluorophore. (2) The hybridization of adsorbed ssDNA probe with its target generates a double stranded DNA (dsDNA). The duplex cannot be adsorbed by CPN due to its rigid conformation and the absence of unpaired DNA bases, leading to an obvious fluorescence enhancement.

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