Communication

Bio-Orthogonal Polymer Coatings for Co-Presentation of Biomolecules

  1. Xiaopei Deng1,
  2. Thomas W. Eyster2,
  3. Yaseen Elkasabi3,
  4. Joerg Lahann4,*

Article first published online: 21 FEB 2012

DOI: 10.1002/marc.201100819

Issue

Macromolecular Rapid Communications

Macromolecular Rapid Communications

Volume 33, Issue 8, pages 640–645, April 23, 2012

Additional Information

How to Cite

Deng, X., Eyster, T. W., Elkasabi, Y. and Lahann, J. (2012), Bio-Orthogonal Polymer Coatings for Co-Presentation of Biomolecules. Macromol. Rapid Commun., 33: 640–645. doi: 10.1002/marc.201100819

Author Information

  1. 1

    Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI 48109, USA

  2. 2

    Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA

  3. 3

    Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA

  4. 4

    Departments of Chemical Engineering, Materials Science and Engineering, Macromolecular Science and Engineering and Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Institute of Functional Interfaces, Karlsruhe Institute of Technology, Hermann-von- Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany

*Departments of Chemical Engineering, Materials Science and Engineering, Macromolecular Science and Engineering and Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA; Institute of Functional Interfaces, Karlsruhe Institute of Technology, Hermann-von- Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.

Publication History

  1. Issue published online: 13 APR 2012
  2. Article first published online: 21 FEB 2012
  3. Manuscript Revised: 19 DEC 2011
  4. Manuscript Received: 30 NOV 2011

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Keywords:

  • biomaterials;
  • biomolecules;
  • coatings;
  • immobilization;
  • surface engineering

Abstract

Controlled presentation of biomolecules on synthetic substrates is an important aspect for biomaterials development. If the immobilization of multiple biomolecules is required, highly efficient orthogonal surface chemistries are needed to ensure the precision of the immobilization. In this communication, chemical vapor deposition (CVD) copolymerization is used to fabricate polymer coatings with controlled ratio of alkyne and pentafluorophenyl ester (Pfp-ester) groups. Cyclic argine-glycine-aspartic acid (cRGD) adhesion peptide and epidermal growth factor (EGF) are immobilized through alkyne–azide cycloaddtion (“click” chemistry) and active ester–amine reaction, respectively. Cell studies with human umbilical vein endothelial cells (HUVEC) and A431 cell lines demonstrate the biological activity of the coimmobilized biomolecules.

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