Preparation, Cellular Internalization, and Biocompatibility of Highly Fluorescent PMMA Nanoparticles

Authors

  • Antje Vollrath,

    1. Laboratory of Organic and Macromolecular Chemistry, Friedrich-Schiller-University Jena, Humboldtstr. 10, 07743 Jena, Germany
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  • David Pretzel,

    1. Laboratory of Organic and Macromolecular Chemistry, Friedrich-Schiller-University Jena, Humboldtstr. 10, 07743 Jena, Germany
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  • Christian Pietsch,

    1. Laboratory of Organic and Macromolecular Chemistry, Friedrich-Schiller-University Jena, Humboldtstr. 10, 07743 Jena, Germany
    2. Dutch Polymer Institute (DPI), Post Office Box 902, Eindhoven 5600 AX, the Netherlands
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  • Igor Perevyazko,

    1. Laboratory of Organic and Macromolecular Chemistry, Friedrich-Schiller-University Jena, Humboldtstr. 10, 07743 Jena, Germany
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  • Stephanie Schubert,

    1. Jena Center for Soft Matter (JCMS), Friedrich-Schiller-University Jena, Humboldtstr. 10, 07743 Jena, Germany
    2. Institute of Pharmacy, Department of Pharmaceutical Technology Friedrich-Schiller-University Jena, Otto-Schott-Str. 41, 07745 Jena, Germany
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  • George M. Pavlov,

    1. Laboratory of Organic and Macromolecular Chemistry, Friedrich-Schiller-University Jena, Humboldtstr. 10, 07743 Jena, Germany
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  • Ulrich S. Schubert

    Corresponding author
    1. Laboratory of Organic and Macromolecular Chemistry, Friedrich-Schiller-University Jena, Humboldtstr. 10, 07743 Jena, Germany
    2. Dutch Polymer Institute (DPI), Post Office Box 902, Eindhoven 5600 AX, the Netherlands
    3. Jena Center for Soft Matter (JCMS), Friedrich-Schiller-University Jena, Humboldtstr. 10, 07743 Jena, Germany
    • Laboratory of Organic and Macromolecular Chemistry, Friedrich-Schiller-University Jena, Humboldtstr. 10, 07743 Jena, Germany.
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Errata

This article is corrected by:

  1. Errata: Correction: Preparation, Cellular Internalization, and Biocompatibility of Highly Fluorescent PMMA Nanoparticles Volume 34, Issue 3, 280, Article first published online: 5 February 2013

Abstract

Methacrylate monomers were functionalized with a 4-hydroxythiazole chromophore and copolymerized with methyl methacrylate via RAFT. Nanoparticles of 120 and 500 nm in size were prepared without using stabilizers/surfactants. For comparative studies, preparative ultracentrifugation was applied for the separation into small and large particle fractions. All suspensions were characterized by DLS, AUC, and SEM and tested regarding their stability during centrifugation and re-suspension, autoclavation, and incubation in cell culture media. In vitro studies with mouse fibroblast cell line and differently sized NP showed a particle uptake into cells. Biocompatibility, non-toxicity, and hemocompatibility were demonstrated using a XTT assay, a live/dead staining, and an erythrocyte aggregation and hemolysis assay.

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