Visible-Light-Mediated Thiol-Ene Hydrogelation Using Eosin-Y as the Only Photoinitiator

Authors

  • Han Shih,

    1. Department of Biomedical Engineering, Indiana University-Purdue University at Indianapolis, Indianapolis, IN 46202, USA
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  • Chien-Chi Lin

    Corresponding author
    1. Department of Biomedical Engineering, Indiana University-Purdue University at Indianapolis, Indianapolis, IN 46202, USA
    • Department of Biomedical Engineering, Indiana University-Purdue University at Indianapolis, Indianapolis, IN 46202, USA.
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Abstract

The utility of visible-light-mediated polymerization in tissue engineering has been limited due to the necessary use of potentially cytotoxic coinitiator and comonomer. Here, we report a visible-light-mediated thiol-ene hydrogelation scheme using eosin-Y as the only photoinitiator. Under visible light exposure, rapid and highly tunable step-growth gelation is achieved using PEG-norbornene and a model cross-linker dithiothreitol. In addition to investigating the gelation kinetics and properties of thiol-ene hydrogels formed by this new gelation scheme, we also report high cytocompatibility of these hydrogels using human mesenchymal stem cells (hMSCs) and pancreatic MIN6 β-cells.

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