Macromolecular Rapid Communications

Cover image for Vol. 31 Issue 13

Special Issue: Synthetic Biomacromolecules for Delivery of Therapeutics, Tissue Engineering, and Imaging

July 1, 2010

Volume 31, Issue 13

Pages 1115–1217

Issue edited by: Y.-Y. Yang, A. J. T. George, K. Kataoka, D. Luo, S. Wang

  1. Cover Picture

    1. Top of page
    2. Cover Picture
    3. Contents
    4. Editorial
    5. Reviews
    6. Communications
    7. Back Cover
    1. Macromol. Rapid Commun. 13/2010

      Mai Sanjoh, Shigehiro Hiki, Yan Lee, Makoto Oba, Kanjiro Miyata, Takehiko Ishii and Kazunori Kataoka

      Article first published online: 30 JUN 2010 | DOI: 10.1002/marc.201090031

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      Front Cover: The cover page presents the integration of an endosome-escaping function into PLys polyplex via electrostatic interaction with a charge-conversion polymer. The charge-conversion polymer converts its charge from negative to positive at acidic endosomal pH to induce endosome disruption, facilitating endosome escape of the PLys polyplex for safe and efficient transfection. Further details can be found in the article by M. Sanjoh, S. Hiki, Y. Lee, M. Oba, K. Miyata, T. Ishii, and K. Kataoka* on page 1181.

  2. Contents

    1. Top of page
    2. Cover Picture
    3. Contents
    4. Editorial
    5. Reviews
    6. Communications
    7. Back Cover
    1. Macromol. Rapid Commun. 13/2010 (pages 1115–1119)

      Article first published online: 30 JUN 2010 | DOI: 10.1002/marc.201090032

  3. Editorial

    1. Top of page
    2. Cover Picture
    3. Contents
    4. Editorial
    5. Reviews
    6. Communications
    7. Back Cover
    1. Macromolecular Synthetic Biomaterials for Delivery of Therapeutics (page 1121)

      Yi-Yan Yang, Andrew J. T. George, Kazunori Kataoka, Dan Luo and Shu Wang

      Article first published online: 30 JUN 2010 | DOI: 10.1002/marc.201000314

  4. Reviews

    1. Top of page
    2. Cover Picture
    3. Contents
    4. Editorial
    5. Reviews
    6. Communications
    7. Back Cover
    1. pH-Responsive Polymers as Gene Carriers (pages 1122–1133)

      In-Kyu Park, Kaushik Singha, Rohidas B. Arote, Yun-Jaie Choi, Won Jong Kim and Chong-Su Cho

      Article first published online: 4 MAY 2010 | DOI: 10.1002/marc.200900867

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      pH-responsive polymers are successfully designed and developed to achieve efficient gene delivery by exploiting the subtle pH differences within the cellular compartments. Installation of appropriate functional as well as structural attributes into the basic polymeric framework could trigger favorable pH-responsive transformations which provide enhanced gene delivery as well as higher biocompatibility.

    2. Intracellular Protein Delivery Systems Formed by Noncovalent Bonding Interactions between Amphipathic Peptide Carriers and Protein Cargos (pages 1134–1141)

      Seong Loong Lo and Shu Wang

      Article first published online: 17 MAY 2010 | DOI: 10.1002/marc.200900934

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      Peptides are emerging as attractive alternatives to polymers and lipids for intracellular protein delivery. Amphipathic peptides are unique among peptide-based protein carriers in their ability to deliver protein cargos into cells without covalent conjugation. β-galactosidase proteins, after mixed with the amphipathic 16K-VTW peptide in solution, are delivered into cells in the presence of serum and able to catalyze the hydrolysis of its substrate X-gal, yielding blue color from the colorless substrate shown in the figure (scale bar = 100 µm).

  5. Communications

    1. Top of page
    2. Cover Picture
    3. Contents
    4. Editorial
    5. Reviews
    6. Communications
    7. Back Cover
    1. Brush-Like Amphoteric Poly[isobutylene-alt-(maleic acid)-graft-oligoethyleneamine)]/DNA Complexes for Efficient Gene Transfection (pages 1142–1147)

      Majad Khan, Goliath Beniah, Nikken Wiradharma, XinDong Guo and Yi Yan Yang

      Article first published online: 1 MAR 2010 | DOI: 10.1002/marc.200900801

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      Novel brush-like amphoteric poly[isobutylene-alt-(maleic acid)-graft-pentaethylenehexamine] has been synthesized. Its nanoscale-sized DNA complexes induce high gene transfection efficiency in various cell lines yet possess little cytotoxicity.

    2. Injectable Biodegradable Poly(ethylene glycol)/RGD Peptide Hybrid Hydrogels for in vitro Chondrogenesis of Human Mesenchymal Stem Cells (pages 1148–1154)

      Shao Qiong Liu, Quan Tian, Lei Wang, James L. Hedrick, James Hoi Po Hui, Yi Yan Yang and Pui Lai Rachel Ee

      Article first published online: 25 MAY 2010 | DOI: 10.1002/marc.200900818

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      Injectable and biodegradable PEG/RGD peptide hybrid hydrogels are synthesized via Michael addition to study the effect of RGD content on chondrogenesis of hMSCs. The incorporation of RGD resulted in enhanced cell viability. Importantly, the presence of RGD at an optimal concentration promoted chondrogenesis of hMSCs.

    3. Efficient Liposomal Nanocarrier-mediated Oligodeoxynucleotide Delivery Involving Dual Use of a Cell-Penetrating Peptide as a Packaging and Intracellular Delivery Agent (pages 1155–1162)

      Phei Er Saw, Young Tag Ko and Sangyong Jon

      Article first published online: 20 MAY 2010 | DOI: 10.1002/marc.200900861

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      A novel liposomal nanocarrier system composing oligoarginine (nonaarginine) as both complexation reagent and as cell penetrating peptide is presented. Nonaarginine, when complexed to oligonucleotide (ODN), greatly increase the encapsulation efficiency of ODN into the liposome (i.e., almost 100% encapsulation). When nonaargine is conjugated to the liposome, it functions as cell penetrating peptide that greatly enhances the uptake of our liposomal formulation in vitro. This 9R-LS holds great promise for a myriad of applications including higher loading of gene or drugs, enhanced cell transfection efficacy and can simultaneously used for tumor-specific targeted delivery in vivo.

    4. Folate-Conjugated Polymer Micelles with pH-Triggered Drug Release Properties (pages 1163–1169)

      Haizheng Zhao, Hoang Hanh Phuoc Duong and Lin Yue Lanry Yung

      Article first published online: 11 MAY 2010 | DOI: 10.1002/marc.200900876

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      The pH-sensitive polymer micelles (left) exhibit stronger fluorescence intensity in the cell nucleus than the PLGA-PEG based micelles (right). This can be attributed to faster drug release from the pH-sensitive micelles at low endosomal pH, which results in a higher flux of drug into the nucleus.

    5. Novel Triblock Oligopeptides as Efficient Nonviral Vectors: Characterisation and Further Insights (pages 1170–1174)

      Wei Yang Seow, Yi-Yan Yang and Andrew J. T. George

      Article first published online: 20 MAY 2010 | DOI: 10.1002/marc.200900883

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      The ex vivo manipulation of corneas prior to transplantation is a promising strategy to prolong graft survival rates. Previously, we designed novel triblock oligopeptides and demonstrated their potential to mediate gene transfer into mouse corneal endothelial cells (MCEC). This study provides further insights into the structure–property relationship of this new class of nonviral vector.

    6. Synthesis of Guanidinium-Modified Hyaluronic Acid Hydrogel (pages 1175–1180)

      Oommen P. Varghese, Marta Kisiel, Elena Martínez-Sanz, Dmitri A. Ossipov and Jöns Hilborn

      Article first published online: 17 MAY 2010 | DOI: 10.1002/marc.200900906

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      A new guanidinylating reagent is developed and employed to synthesise dually functionalised hyaluronan possessing guanidinium and crosslinkable hydrazide moieties. Hydrozone crosslinked hydrogel is formed within 50 s with improved mechanical properties. This derivative has minimal toxicity (∼80% cell viability at 2.5 × 10−3M concentration), forms polyelectrolyte complex with plasmid DNA and shows DNA transfection to CD44 positive cells.

    7. pDNA/poly(L-lysine) Polyplexes Functionalized with a pH-Sensitive Charge-Conversional Poly(aspartamide) Derivative for Controlled Gene Delivery to Human Umbilical Vein Endothelial Cells (pages 1181–1186)

      Mai Sanjoh, Shigehiro Hiki, Yan Lee, Makoto Oba, Kanjiro Miyata, Takehiko Ishii and Kazunori Kataoka

      Article first published online: 20 MAY 2010 | DOI: 10.1002/marc.201000056

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      The enhanced gene expression of poly(L-lysine) polyplex was successfully achieved by integration of charge-conversional polymer (CCP). CCP showed charge-conversional property in response to endosomal pH, leading to outstanding endosomal escape of the polyplex to achieve high transfection efficiency against endothelial cells without toxicity.

    8. Delivery of Anticancer Drugs Using Polymeric Micelles Stabilized by Hydrogen-Bonding Urea Groups (pages 1187–1192)

      Jeremy P. K. Tan, Sung Ho Kim, Fredrik Nederberg, Kazuki Fukushima, Daniel J. Coady, Alshakim Nelson, Yi Yan Yang and James L. Hedrick

      Article first published online: 22 JUN 2010 | DOI: 10.1002/marc.201000105

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      Hydrogen-bonding urea groups were implemented within the hydrophobic domain of amphiphilic micelles.This non-conventional approach improved micelle stability and doxirubicin loading capacity. The drug-loaded micelles were then shown to effectively kill all cell lines tested, while the polymeric carrier exhibited no cytotoxicity.

    9. Formation and Degradation of Biodegradable Triple-Layered Microparticles (pages 1193–1200)

      Wei Li Lee, Meiju Hong, Effendi Widjaja and Say Chye Joachim Loo

      Article first published online: 9 MAR 2010 | DOI: 10.1002/marc.200900811

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      Triple-layered PLGA/PLLA/EVA (shell to core) microparticles are fabricated through a simple, economical and reliable one-step solvent evaporation technique. Compared to the single- and double-layered microparticles, triple-layered microparticles possess unique structural attributes and hydrolytic degradation characteristics. These triple-walled microparticles could then provide a means of controlling drug release.

    10. Core–Shell–Corona Micelle Stabilized by Reversible Cross-Linkage for Intracellular Drug Delivery (pages 1201–1206)

      Yu-Cai Wang, Yang Li, Tian-Meng Sun, Meng-Hua Xiong, Juan Wu, Yi-Yan Yang and Jun Wang

      Article first published online: 19 MAR 2010 | DOI: 10.1002/marc.200900863

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      Biodegradable polymeric micelles with core–shell–corona structures are prepared. Selective cross-linking of the shell layer stabilizes the micelles against dilution and retards drug release in a non-reductive environment. However, the release is accelerated intracellularly, which results in enhanced cytotoxicity to A549 cancer cells.

    11. Photocrosslinked DNA Nanospheres for Drug Delivery (pages 1207–1211)

      Young Hoon Roh, Jong Bum Lee, Shawn J. Tan, Bojeong Kim, Hyeongsu Park, Edward J. Rice and Dan Luo

      Article first published online: 25 MAY 2010 | DOI: 10.1002/marc.200900872

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      Novel monodisperse and tunable DNA nanospheres have been generated via rapid photocrosslinking of photoreactive branched DNA monomers. This paper describes the synthesis and characterization of photocrosslinked DNA nanospheres that may have potential applications as drug delivery carriers.

    12. Design and Evaluation of Peptide Amphiphiles with Different Hydrophobic Blocks for Simultaneous Delivery of Drugs and Genes (pages 1212–1217)

      Nikken Wiradharma, Yen Wah Tong and Yi-Yan Yang

      Article first published online: 22 JUN 2010 | DOI: 10.1002/marc.200900935

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      Cationic amphiphilic triblock oligopeptides with different amino acid compositions in the hydrophobic block have been designed and synthesized. Such compositions have a significant effect on micellization, drug loading and gene transfection. The peptide with the optimized composition results in nanosized micelles, high drug loading capacity and efficient gene transfection. Importantly, it can deliver drug and gene simultaneously, which further enhances gene transfection.

  6. Back Cover

    1. Top of page
    2. Cover Picture
    3. Contents
    4. Editorial
    5. Reviews
    6. Communications
    7. Back Cover
    1. Macromol. Rapid Commun. 13/2010

      Phei Er Saw, Young Tag Ko and Sangyong Jon

      Article first published online: 30 JUN 2010 | DOI: 10.1002/marc.201090033

      Thumbnail image of graphical abstract

      Back Cover: The figure shows a liposomal nanocarrier (9R-LS) encapsulating a nonaarginine oligodeoxynucleotide (9R/ODN) complex. After 9R has facilitated the uptake of 9R-LS, the 9R/ODN complex is dissociated in the cytoplasm. The ODN blocks the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) binding to the promoter region of target genes, thus shutting down transcription of proliferation-related genes. Further details can be found in the article by P. E. Saw, Y. T. Ko,* S. Jon*on page 1155.

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